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Title: Comparisons of the effects of TCDD and hydrocortisone on growth factor expression provide insight into their interaction in the embryonic mouse palate

Abstract

Cleft palate (CP) can be induced in embryonic mice by a wide range of compounds, including glucocorticoids and 2,3,7,8-tyetrachlorodibenzo-p-dioxin (TCDD). Hydrocortisone (HC), a glucocorticoid, retards embryonic growth producing small palatal shelves, while TCDD exposure blocks the fusion of normally sized shelves. TCDD induction of CP involves altered differentiation of the medial epithelial cells. Recent studies indicate that growth factors such as EGF, TGF-alpha, TGF-beta1, and TGF-beta2 are involved in palatogenesis, regulating proliferation, differentiation, and extracellular matrix production. A synergism has been observed between HC and TCDD in which doses too low to induce CP alone are able to produce >90% incidence when coadministered. In the present study a standard teratology protocol was performed in C57BL/6N mice to examine the synergism at doses lower than those previously published. Data from the study indicate synergistic interactions at doses as low as 3 micrograms TCDD/kg + 1 mg HC/kg. This extreme sensitivity suggests the involvement of a receptor-mediated mechanism possibly resulting in altered regulation of gene expression. (Copyright (c) 1992 Wiley-Liss, Inc.)

Authors:
; ;
Publication Date:
Research Org.:
Environmental Protection Agency, Research Triangle Park, NC (United States). Health Effects Research Lab.
OSTI Identifier:
5512560
Report Number(s):
PB-92-158641/XAB; EPA-600/J-92/108
Resource Type:
Technical Report
Resource Relation:
Other Information: Pub. in Teratology 45, n1 p35-53 Jan 92. Prepared in cooperation with National Inst. of Environmental Health Sciences, Research Triangle Park, NC
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DIOXIN; TERATOGENESIS; HYDROCORTISONE; BIOLOGICAL EFFECTS; ORAL CAVITY; MORPHOLOGICAL CHANGES; CELL DIFFERENTIATION; ELECTRON MICROSCOPY; EMBRYOS; EPITHELIUM; GENE REGULATION; GROWTH FACTORS; MICE; SYNERGISM; ADRENAL HORMONES; ANIMAL TISSUES; ANIMALS; BODY; CORTICOSTEROIDS; DIGESTIVE SYSTEM; GLUCOCORTICOIDS; HETEROCYCLIC COMPOUNDS; HORMONES; HYDROXY COMPOUNDS; KETONES; MAMMALS; MICROSCOPY; MITOGENS; ORGANIC COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; PREGNANES; PROTEINS; RODENTS; STEROID HORMONES; STEROIDS; TISSUES; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Abbott, B D, Harris, M W, and Birnbaum, L S. Comparisons of the effects of TCDD and hydrocortisone on growth factor expression provide insight into their interaction in the embryonic mouse palate. United States: N. p., 1992. Web.
Abbott, B D, Harris, M W, & Birnbaum, L S. Comparisons of the effects of TCDD and hydrocortisone on growth factor expression provide insight into their interaction in the embryonic mouse palate. United States.
Abbott, B D, Harris, M W, and Birnbaum, L S. 1992. "Comparisons of the effects of TCDD and hydrocortisone on growth factor expression provide insight into their interaction in the embryonic mouse palate". United States.
@article{osti_5512560,
title = {Comparisons of the effects of TCDD and hydrocortisone on growth factor expression provide insight into their interaction in the embryonic mouse palate},
author = {Abbott, B D and Harris, M W and Birnbaum, L S},
abstractNote = {Cleft palate (CP) can be induced in embryonic mice by a wide range of compounds, including glucocorticoids and 2,3,7,8-tyetrachlorodibenzo-p-dioxin (TCDD). Hydrocortisone (HC), a glucocorticoid, retards embryonic growth producing small palatal shelves, while TCDD exposure blocks the fusion of normally sized shelves. TCDD induction of CP involves altered differentiation of the medial epithelial cells. Recent studies indicate that growth factors such as EGF, TGF-alpha, TGF-beta1, and TGF-beta2 are involved in palatogenesis, regulating proliferation, differentiation, and extracellular matrix production. A synergism has been observed between HC and TCDD in which doses too low to induce CP alone are able to produce >90% incidence when coadministered. In the present study a standard teratology protocol was performed in C57BL/6N mice to examine the synergism at doses lower than those previously published. Data from the study indicate synergistic interactions at doses as low as 3 micrograms TCDD/kg + 1 mg HC/kg. This extreme sensitivity suggests the involvement of a receptor-mediated mechanism possibly resulting in altered regulation of gene expression. (Copyright (c) 1992 Wiley-Liss, Inc.)},
doi = {},
url = {https://www.osti.gov/biblio/5512560}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1992},
month = {Wed Jan 01 00:00:00 EST 1992}
}

Technical Report:
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