Structural features of the human C3 gene: Intron/exon organization, transcriptional start site, and promoter region sequence

Journal Article · Tue Jan 29 00:00:00 EST 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00218a029· OSTI ID:5510477

Vik, D P; Amiguet, P; Moffat, G J; Fey, M; Amiguet-Barras, F; Tack, B F ^[1]; Wetsel, R A ^[2]

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1. Scripps Clinic and Research Foundation, La Jolla, CA (USA)
2. Washington Univ., St. Louis, MO (USA)

The third component of human complement (C3) is a key molecule in the activation of the complement cascade. C3 cDNA fragments were used to identify seven cosmid clones that covered all but 1 kilobase pair (kb) of the C3 gene. The remainder of the gene was cloned by using the polymerase chain reaction. These clones were used to identify the interon/exon boundaries and to map the gene. The C3 gene is 42 kb in length and comprises 41 exons ranging in size from 52 to 213 base pairs (bp). The transcription start site was identified by primer extension, and approximately 1 kb of DNA upstream of this site was sequenced. Putative TATA and CAAT boxes were identified along with a number of regions that shared homology with known regulatory sequences. These include responsive elements for interferon-{gamma}, interleukin-6, nuclear factor kB, estrogen, glucocorticoids and thyroid hormone. Several of these agents have been shown to affect C3 synthesis and mRNA levels. The sizes of the exons in C3 were compared to those of C4 and {alpha}{sub 2}-macroglobulin ({alpha}{sub 2}M). Thirty-nine of 41 exons in C4 were found to be of similar size to the analogous ones in C3, and two-thirds of those in {alpha}{sub 2}M were also similarly sized, supporting the hypothesis that these genes arose from a common ancestor.

• OSTI ID: 5510477
• Journal Information: Biochemistry; (United States), Vol. 30:4; ISSN 0006-2960
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
COMPLEMENT
GENE REGULATION
GENES
DNA SEQUENCING
DNA POLYMERASES
ELECTROPHORESIS
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PHOSPHORUS 32
PROMOTERS
TRANSCRIPTION
ANIMALS
BETA DECAY RADIOISOTOPES
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PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PRIMATES
PROTEINS
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RNA
STRUCTURAL CHEMICAL ANALYSIS
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550201* - Biochemistry- Tracer Techniques