Vasoactive intestinal peptide binding sites and fibers in the brain of the pigeon Columba livia: An autoradiographic and immunohistochemical study

Journal Article · Fri Mar 15 00:00:00 EST 1991 · Journal of Comparative Neurology; (United States)

DOI:https://doi.org/10.1002/cne.903050304· OSTI ID:5510146

Hof, P R; Dietl, M M; Charnay, Y; Martin, J L; Bouras, C; Palacios, J M; Magistretti, P J ^[1]

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1. Fishberg Research Center for Neurobiology, Mount Sinai School of Medicine, New York, NY (USA)

The distribution of vasoactive intestinal peptide (VIP) binding sites in the pigeon brain was examined by in vitro autoradiography on slide-mounted sections. A fully characterized monoiodinated form of VIP, which maintains the biological activity of the native peptide, was used throughout this study. The highest densities of binding sites were observed in the hyperstriatum dorsale, archistriatum, auditory field L of neostriatum, area corticoidea dorsolateralis and temporo-parieto-occipitalis, area parahippocampalis, tectum opticum, nucleus dorsomedialis anterior thalami, and in the periventricular area of the hypothalamus. Lower densities of specific binding occurred in the neostriatum, hyperstriatum ventrale and nucleus septi lateralis, dorsolateral area of the thalamus, and lateral and posteromedial hypothalamus. Very low to background levels of VIP binding were detected in the ectostriatum, paleostriatum primitivum, paleostriatum augmentatum, lobus parolfactorius, nucleus accumbens, most of the brainstem, and the cerebellum. The distribution of VIP-containing fibers and terminals was examined by indirect immunofluorescence using a polyclonal antibody against porcine VIP. Fibers and terminals were observed in the area corticoidea dorsolateralis, area parahippocampalis, hippocampus, hyperstriatum accessorium, hyperstriatum dorsale, archistriatum, tuberculum olfactorium, nuclei dorsolateralis and dorsomedialis of the thalamus, and throughout the hypothalamus and the median eminence. Long projecting fibers were visualized in the tractus septohippocampalis. In the brainstem VIP immunoreactive fibers and terminals were observed mainly in the substantia grisea centralis, fasciculus longitudinalis medialis, lemniscus lateralis, and in the area surrounding the nuclei of the 7th, 9th, and 10th cranial nerves.

• OSTI ID: 5510146
• Journal Information: Journal of Comparative Neurology; (United States), Vol. 305:3; ISSN 0021-9967
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BRAIN
AUTORADIOGRAPHY
PEPTIDES
RECEPTORS
TISSUE DISTRIBUTION
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
FLUORESCENCE
PIGEONS
ANIMALS
BIRDS
BODY
CENTRAL NERVOUS SYSTEM
DISTRIBUTION
KINETICS
LUMINESCENCE
MEMBRANE PROTEINS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PROTEINS
REACTION KINETICS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques