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Title: Atrial natriuretic factor: radioimmunoassay and effects on adrenal and pituitary glands

Abstract

A simple and sensitive radioimmunoassay was developed for measurement of immunoreactive atrial natriuretic factor (IR-ANF) in rat and human plasma and in rat atria. The two atria contain about 20 ..mu..g ANF per rat. The right atrium contained 2.5 times more ANF than did the left. Ether anesthesia and morphine markedly increased IR-ANF in rat plasma. The concentration of IR-ANF in plasma of clinically normal human subjects was 65.3 +/- 2.5 pg/ml. Paroxysmal tachycardia and rapid atrial pacing significantly increased IR-ANF in human plasma. Two- to seven-fold higher concentrations were found in coronary sinus blood than in the peripheral circulation. In the plasma of rats and humans, circulating ANF is probably a small-molecular-weight peptide. ANF acts on the adrenal and the pituitary. ANF inhibits aldosterone secretion from rat zona glomerulosa and steroid secretion by bovine adrenal zona glomerulosa and fasciculata. ANF stimulated the basal secretion of arginine vasopressin (AVP) in vitro and inhibited KCl-stimulated release of AVP.

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Clinical Research Institute of Montreal, Quebec
OSTI Identifier:
5509459
Resource Type:
Journal Article
Journal Name:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
Additional Journal Information:
Journal Volume: 45:7
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ALDOSTERONE; SECRETION; PEPTIDES; BIOLOGICAL EFFECTS; RADIOIMMUNOASSAY; STEROIDS; VASOPRESSIN; ADRENAL GLANDS; BLOOD PLASMA; IMMUNE REACTIONS; INHIBITION; MAN; PITUITARY GLAND; RATS; ADRENAL HORMONES; ALDEHYDES; ANIMALS; BIOLOGICAL MATERIALS; BLOOD; BODY; BODY FLUIDS; CORTICOSTEROIDS; ENDOCRINE GLANDS; GLANDS; HORMONES; HYDROXY COMPOUNDS; IMMUNOASSAY; IMMUNOLOGY; ISOTOPE APPLICATIONS; KETONES; MAMMALS; MATERIALS; MINERALOCORTICOIDS; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; PITUITARY HORMONES; PREGNANES; PRIMATES; PROTEINS; RADIOASSAY; RADIOIMMUNOLOGY; RODENTS; STEROID HORMONES; TRACER TECHNIQUES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Gutkowska, J, Horky, K, Schiffrin, E L, Thibault, G, Garcia, R, De Lean, A, Hamet, P, Tremblay, J, Anand-Srivastava, M B, and Januszewicz, P. Atrial natriuretic factor: radioimmunoassay and effects on adrenal and pituitary glands. United States: N. p., 1986. Web.
Gutkowska, J, Horky, K, Schiffrin, E L, Thibault, G, Garcia, R, De Lean, A, Hamet, P, Tremblay, J, Anand-Srivastava, M B, & Januszewicz, P. Atrial natriuretic factor: radioimmunoassay and effects on adrenal and pituitary glands. United States.
Gutkowska, J, Horky, K, Schiffrin, E L, Thibault, G, Garcia, R, De Lean, A, Hamet, P, Tremblay, J, Anand-Srivastava, M B, and Januszewicz, P. 1986. "Atrial natriuretic factor: radioimmunoassay and effects on adrenal and pituitary glands". United States.
@article{osti_5509459,
title = {Atrial natriuretic factor: radioimmunoassay and effects on adrenal and pituitary glands},
author = {Gutkowska, J and Horky, K and Schiffrin, E L and Thibault, G and Garcia, R and De Lean, A and Hamet, P and Tremblay, J and Anand-Srivastava, M B and Januszewicz, P},
abstractNote = {A simple and sensitive radioimmunoassay was developed for measurement of immunoreactive atrial natriuretic factor (IR-ANF) in rat and human plasma and in rat atria. The two atria contain about 20 ..mu..g ANF per rat. The right atrium contained 2.5 times more ANF than did the left. Ether anesthesia and morphine markedly increased IR-ANF in rat plasma. The concentration of IR-ANF in plasma of clinically normal human subjects was 65.3 +/- 2.5 pg/ml. Paroxysmal tachycardia and rapid atrial pacing significantly increased IR-ANF in human plasma. Two- to seven-fold higher concentrations were found in coronary sinus blood than in the peripheral circulation. In the plasma of rats and humans, circulating ANF is probably a small-molecular-weight peptide. ANF acts on the adrenal and the pituitary. ANF inhibits aldosterone secretion from rat zona glomerulosa and steroid secretion by bovine adrenal zona glomerulosa and fasciculata. ANF stimulated the basal secretion of arginine vasopressin (AVP) in vitro and inhibited KCl-stimulated release of AVP.},
doi = {},
url = {https://www.osti.gov/biblio/5509459}, journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 45:7,
place = {United States},
year = {Sun Jun 01 00:00:00 EDT 1986},
month = {Sun Jun 01 00:00:00 EDT 1986}
}