The effects of ionophores and metabolic inhibitors on methanogenesis and energy-related properties of Methanobacterium bryantii

Journal Article · Mon Aug 01 00:00:00 EDT 1983 · Arch. Biochem. Biophys.; (United States)

DOI:https://doi.org/10.1016/0003-9861(83)90004-8· OSTI ID:5505268

Jarrell, K F; Sprott, G D

The effects of numerous ionophores and inhibitors were tested on methane synthesis, intracellular ATP and potassium concentrations, and the proton motive force of the methanogenic archaebacterium Methanobacterium bryantii. M. bryantii had an internal pH near 6.8 (and hence little delta pH during growth) with an electrical potential of --127 mV in growth medium and --105 mV in a pH 6.5 buffer. The study has identified agents which, in M. bryantii, can effectively cause a decline of intracellular ATP (gramicidin, acetylene) and potassium concentrations (gramicidin, nigericin), inhibit methane synthesis (acetylene, gramicidin, nigericin, triphenylmethylphosphonium bromide), eliminate the electrical potential (high extracellular potassium ion concentrations), and dissipate artificially imposed, inside alkaline, pH gradients (monensin, nigericin, carbonyl cyanide m-chlorophenylhydrazone). Carbonyl cyanide m-chlorophenylhydrazone was generally ineffective in media or buffers reduced with cysteine-sulfide but could be effective in cysteine-free solutions reduced with hydrogen sulfide.

• Research Organization: Division of Biological Sciences, National Research Council of Canada, Ottawa, Ontario
• OSTI ID: 5505268
• Journal Information: Arch. Biochem. Biophys.; (United States), Vol. 225:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
CELL MEMBRANES
BIOELECTRICITY
METHANE
BIOSYNTHESIS
METHANOGENIC BACTERIA
INHIBITION
ACETYLENE
ANTIBIOTICS
ATP
POTASSIUM
ALKALI METALS
ALKANES
ALKYNES
ANTI-INFECTIVE AGENTS
BACTERIA
CELL CONSTITUENTS
DRUGS
ELECTRICITY
ELEMENTS
HYDROCARBONS
MEMBRANES
METALS
MICROORGANISMS
NUCLEOTIDES
ORGANIC COMPOUNDS
SYNTHESIS
550200* - Biochemistry
550700 - Microbiology
090122 - Hydrocarbon Fuels- Preparation from Wastes or Biomass- (1976-1989)