Characterization of additional rabbit IgM allotypes and the effect of suppression of a VH locus allotypes on the expression of n C. mu. locus allotype

Conference · Fri Jan 01 00:00:00 EST 1982 · J. Immunol.; (United States)

OSTI ID:5505110

Gilman-Sachs, A; Roux, K H; Horing, W J; Dray, S

Anti-allotype antisera were produced that identified eight rabbit IgM allotypic specificities, n80, n81, n82, n83, n84, n85, n86, and n87. The n locus C..mu.. genes controlling these IgM allotypic specificities are closely linked to the a (VH subgroup) locus. The genes controlling these allotypic specificities were found to be in the heavy chain chromosomal region and were assigned to 11 haplotypes present in our rabbit colony. The n locus and a locus genes appeared in the haplotypes in six combinations: a/sup 1/n/sup 81/, a/sup 2/n/sup 81,n87/, a/sup 1/n/sup 80,83/, a/sup 2/n/sup 80,82,87/, a/sup 3/n/sup 81,84,85/ and a/sup 3/n/sup 80,84,86,87/. By radioprecipitation analysis, 70 to 80% of serum IgM reacts with the antiserum directed to each n locus allotypic specificity found encoded in one haplotype; thus, each allotypic specificity of the haplotype is present on the same IgM molecule. When sera from a locus allotype-suppressed homozygous rabbits were tested for expression of each n locus allotypic specificity, n80, n81, and n87 were still expressed, whereas n82, n83, n84, n85, and n86 were not. These data provide direct evidence that some IgM specificities are expressed independently of the a locus (i.e., ''true''), and other s are dependent on the expression of an a locus specificity (i.e., conformational). The expression of the ''true'' allotypic specificities probably reflects genetic control of the germline C..mu.. gene, and the expression of ''conformationally dependent'' allotypic specificities probably reflects the interaction of VH and C..mu.. gene segments. This distinction is important and must be recognized when evaluating the genetics and structure of the IgM molecule.

• Research Organization: Univ. of Illinois Medical Center, Chicago
• OSTI ID: 5505110
• Journal Information: J. Immunol.; (United States), Vol. 128:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
IMMUNOGLOBULINS
GENETIC CONTROL
LABELLING
GENES
IODINE 125
RABBITS
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SEPARATION PROCESSES
ANIMALS
BETA DECAY RADIOISOTOPES
CONTROL
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
INTERMEDIATE MASS NUCLEI
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ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
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PROTEINS
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TRACER TECHNIQUES
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550401* - Genetics- Tracer Techniques