Multiple sites of methylation in the methyl accepting chemotaxis proteins of Escherichia coli
The methyl-accepting chemotaxis proteins (MCP) of E coli show at least five bands when subjected to SDS-gel electrophoresis. The intensity of the individual bands varies depending on the environment of the cells before solubilization. The faster migrating bands are enhanced following attractant stimulation, whereas the slower migrating bands are enhanced following attractant dilution or repellent increase. The time scale of these intensity changes is similar to that for adaptation of the behavioral response in these cells suggesting that methylation of the MCP is involved in producing these bands. Peptide mapping experiments show three methylated peptides in both MCP I and MCP II. These results suggest multiple sites of methylation, which are responsible, at least in part, for the observed multiple bands of the MCPs.
- Research Organization:
- Institute of Molecular Biology, University of Oregon, Eugene
- OSTI ID:
- 5504694
- Journal Information:
- Prog. Clin. Biol. Res.; (United States), Vol. 63
- Country of Publication:
- United States
- Language:
- English
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