skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Radiometric--microbiologic assay of vitamin B-6: application to food analysis

Abstract

A radiometric microbiologic assay for vitamin B-6 was applied to food analysis. The method was shown to be specific, reproducible and simpler than the standard turbidimetric microbiologic technique. The analysis of seven commercially available breakfast cereals was compared to a high performance liquid chromatography method. Three out of the seven cereals agreed when assayed with both methods (P greater than 0.1). Four cereals, however, differed in value considerably (P less than 0.05). Further studies are required to determine whether these differences were due to different extraction procedures used. The study showed that the new radiometric-microbiologic method can be used to measure total vitamin B-6 or, combined with a column separation procedure, to analyze for specific forms of the vitamin.

Authors:
; ;
Publication Date:
Research Org.:
Department of Environmental Health Sciences, The Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD
OSTI Identifier:
5503750
Resource Type:
Journal Article
Journal Name:
J. Nutr.; (United States)
Additional Journal Information:
Journal Volume: 111:11
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 60 APPLIED LIFE SCIENCES; FOOD; CHEMICAL COMPOSITION; PYRIDOXINE; RADIOASSAY; COMPARATIVE EVALUATIONS; LIQUID COLUMN CHROMATOGRAPHY; AZINES; CHROMATOGRAPHY; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIDINES; SEPARATION PROCESSES; VITAMIN B GROUP; VITAMINS; 550601* - Medicine- Unsealed Radionuclides in Diagnostics; 553001 - Agriculture & Food Technology- Tracer Techniques- (-1987)

Citation Formats

Guilarte, T R, Shane, B, and McIntyre, P A. Radiometric--microbiologic assay of vitamin B-6: application to food analysis. United States: N. p., 1981. Web.
Guilarte, T R, Shane, B, & McIntyre, P A. Radiometric--microbiologic assay of vitamin B-6: application to food analysis. United States.
Guilarte, T R, Shane, B, and McIntyre, P A. 1981. "Radiometric--microbiologic assay of vitamin B-6: application to food analysis". United States.
@article{osti_5503750,
title = {Radiometric--microbiologic assay of vitamin B-6: application to food analysis},
author = {Guilarte, T R and Shane, B and McIntyre, P A},
abstractNote = {A radiometric microbiologic assay for vitamin B-6 was applied to food analysis. The method was shown to be specific, reproducible and simpler than the standard turbidimetric microbiologic technique. The analysis of seven commercially available breakfast cereals was compared to a high performance liquid chromatography method. Three out of the seven cereals agreed when assayed with both methods (P greater than 0.1). Four cereals, however, differed in value considerably (P less than 0.05). Further studies are required to determine whether these differences were due to different extraction procedures used. The study showed that the new radiometric-microbiologic method can be used to measure total vitamin B-6 or, combined with a column separation procedure, to analyze for specific forms of the vitamin.},
doi = {},
url = {https://www.osti.gov/biblio/5503750}, journal = {J. Nutr.; (United States)},
number = ,
volume = 111:11,
place = {United States},
year = {Sun Nov 01 00:00:00 EST 1981},
month = {Sun Nov 01 00:00:00 EST 1981}
}