Identification of a hypoxic population of bone marrow cells
A technique using collagenase has been devised to release and separate, with reproducibility, hematopoietic cells (HC) from various microenvironments of mouse femurs. HC were assayed by an in vitro gel culture technique used traditionally to score granulocyte-macrophage precursor cells (CFU-C). CFU-C which resided in the medullary cavity and endosteal regions were sensitive to ionizing radiation and resistant to misonidazole (MISO) cytotoxicity. CFU-C which resided within the compact bone were resistant to ionizing radiation and sensitive to the cytotoxic action of MISO. These results suggest that HC which reside in the bone are hypoxic and retain clonogenic potential. When animals were exposed to various treatments with MISO followed by myelotoxic doses of cyclophosphamide (CTX) or total body irradiation (TBI), the LD/sub 50/ of both agents was significantly reduced. This result suggests that a hypoxic component of HC could be important in the regenerative process within the marrow after such myelotoxic trauma.
- Research Organization:
- Univ. of Alberta, Canada
- OSTI ID:
- 5502552
- Journal Information:
- Int. J. Radiat. Oncol., Biol. Phys.; (United States), Vol. 9:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
BONE MARROW CELLS
RADIOSENSITIVITY
ANOXIA
CFU
CHEMOTHERAPY
ENDOXAN
IN VITRO
IONIZING RADIATIONS
MISONIDAZOLE
RADIOTHERAPY
ALKYLATING AGENTS
ANIMAL CELLS
ANTINEOPLASTIC DRUGS
AZOLES
CONNECTIVE TISSUE CELLS
DRUGS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
IMMUNOSUPPRESSIVE DRUGS
MEDICINE
NUCLEAR MEDICINE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIATIONS
RADIOLOGY
RADIOSENSITIZERS
SOMATIC CELLS
THERAPY
560121* - Radiation Effects on Cells- External Source- (-1987)
550603 - Medicine- External Radiation in Therapy- (1980-)