Lasting engraftment of histoincompatible bone marrow cells in dogs
Conditioning protocols were tested for their efficacy in increasing the incidence of engraftment of histoincompatible dog bone marrow cells. Cyclophosphamide and total body irradation (TBI), Corynebacterium parvum and TBI, a 3- or 5-day delayed transfusion of bone marrow cells after TBI, or an increase in the number of donor bone marrow cells or lymphocytes appeared to be ineffective. These protocols were previously reported to promote recovery of splenic hemopoiesis in mice in short-term assays. The noted discrepancy between studies with mice and dogs invalidated allogeneic resistance as measured in the mouse spleen assay as a model for bone marrow allograft rejection. Intravenous treatment with silica particles or L-asparaginase did improve the engraftment rate after 7.5 Gy TBI. Low efficiency and significant extra toxicity restrict the applicability of these procedures. The most promising conditioning schedule found appeared to be two fractions of 6.0 Gy TBI separated by a 72-hr interval. Prolonged survival was noted after transplantation of bone marrow cells from a one-DLA haplo-type-mismatched donor. Possibilities for further improvement of this protocol are discussed.
- OSTI ID:
- 5502214
- Journal Information:
- Transplantation; (United States), Vol. 31:5
- Country of Publication:
- United States
- Language:
- English
Similar Records
Cyclosporin A and methotrexate in canine marrow transplantation: engraftment, graft-versus-host disease, and induction of intolerance
Marrow grafts between phenotypically DLA-identical and haploidentical unrelated dogs: additional antigens controlling engraftment are not detected by cell-mediated lympholysis
Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
59 BASIC BIOLOGICAL SCIENCES
BONE MARROW CELLS
GRAFTS
DOGS
BIOLOGICAL RADIATION EFFECTS
GRAFT-HOST REACTION
RADIOSENSITIVITY
COMPARATIVE EVALUATIONS
CORYNEBACTERIUM PARVUM
ENDOXAN
FRACTIONATED IRRADIATION
MICE
WHOLE-BODY IRRADIATION
ALKYLATING AGENTS
ANIMAL CELLS
ANIMALS
BACTERIA
BIOLOGICAL EFFECTS
CONNECTIVE TISSUE CELLS
DRUGS
EXTERNAL IRRADIATION
IMMUNOSUPPRESSIVE DRUGS
IRRADIATION
MAMMALS
MICROORGANISMS
RADIATION EFFECTS
RODENTS
SOMATIC CELLS
TRANSPLANTS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals
550603 - Medicine- External Radiation in Therapy- (1980-)
551000 - Physiological Systems