Formaldehyde-induced acentric chromosome fragments and chromosome stickiness in Chortophaga neuroblasts
Embryos of the grasshopper Chortophaga viridifasciata were exposed in vitro to formaldehyde (FA), as formalin, at concentrations ranging from 10/sup -8/ M (0.0003 ppm) to 10/sup -3/ M (30 ppm) at 38/sup 0/C. A low frequency of distinct acentric chromosome fragments was observed in the neuroblasts after 1 hr exposure to 7.5 x 10/sup -4/ or 10/sup -3/ M FA plus 3 hr recovery, but not at lower concentrations, even with 4 hr exposure. Neuroblasts with sticky chromosomes were observed at 10/sup -4/, 7.5 x 10/sup -4/, and 10/sup -3/ M FA, the percent of cells with slight, moderate, or severe stickiness varying with FA concentrations. Fragments were associated with the sticky chromosomes. It is concluded that the distinct acentric fragments induced by FA result from breakage at a single sticky point (slight stickiness) between separating sister chromatids. The chromosome effects observed probably result from the action of daughter products that are formed by the interaction of FA with culture medium components, especially the fetal calf serum.
- Research Organization:
- Univ. of Texas, Dallas
- OSTI ID:
- 5498590
- Journal Information:
- Environ. Mutagen.; (United States), Vol. 8:3
- Country of Publication:
- United States
- Language:
- English
Similar Records
X-RAY INDUCED INCORPORATION OF TRITIATED THYMIDINE INTO GRASSHOPPER NEUROBLAST CHROMOSOMES
Mitotic effects of monochromatic ultraviolet radiation at 225, 265, and 280 nm on eleven stages of the cell cycle of the grasshopper neuroblast in culture. II. Changes in progression rate and cell sequence between the stage irradiated and nuclear membrane breakdown
Related Subjects
FORMALDEHYDE
GENETIC EFFECTS
NERVE CELLS
CHROMOSOMAL ABERRATIONS
EMBRYOS
GRASSHOPPERS
ALDEHYDES
ANIMAL CELLS
ANIMALS
ARTHROPODS
BIOLOGICAL EFFECTS
INSECTS
INVERTEBRATES
MUTATIONS
ORGANIC COMPOUNDS
ORTHOPTERA
SOMATIC CELLS
560304* - Chemicals Metabolism & Toxicology- Invertebrates- (-1987)