Normal macrophage function in copper deficient mice
Copper deficiency (-Cu) was produced in C57 BL and C58 mice by feeding a low copper diet (modified AIN-76A) from birth. Mice given supplemental copper in the drinking water (+Cu) served as controls. Copper status was monitored by assay of ceruloplasmin (CP) activity. Macrophages (M0) were obtained from matched +Cu and -Cu male 7 week-old mice by peritoneal lavage 3 days after thioglycollate stimulation. M0 were assayed in terms of lipopolysaccharide-induced hexose monophosphate shunt activity by monitoring /sup 14/CO/sub 2/ production from (1-/sup 14/C)-glucose and by the determination of phagocytic index using fluorescein labelled latex bead ingestion. M0 from -Cu mice were equivalent to those of +Cu mice in both these parameters. However, superoxide dismutase and cytochrome oxidase activities were both significantly lower in -Cu M0, confirming a functional copper deficiency. Previous results from this laboratory have shown that -Cu mice have a decreased antibody response to sheep erythrocyte antigens and a diminished reactivity to B and T cell mitogens. These immunological insufficiencies appear to be proportional to the severity of copper depletion as determined by CP levels. Furthermore, -Cu lymphocytes exhibit depressed mixed lymphocyte reactivity consistent with alterations at the membrane surface. The present results suggest that M0/monocytes are less severely affected than lymphocytes in copper deficiency states.
- Research Organization:
- Univ. of Minnesota, Duluth
- OSTI ID:
- 5498045
- Report Number(s):
- CONF-8604222-; TRN: 86-026832
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
COPPER
NUTRITIONAL DEFICIENCY
MACROPHAGES
BIOLOGICAL FUNCTIONS
OXIDOREDUCTASES
ENZYME ACTIVITY
SUPEROXIDE DISMUTASE
CARBON 14 COMPOUNDS
CARBON DIOXIDE
DIET
GLUCOSE
LYMPHOCYTES
MICE
TOXICITY
TRACER TECHNIQUES
ALDEHYDES
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARBON COMPOUNDS
CARBON OXIDES
CHALCOGENIDES
CONNECTIVE TISSUE CELLS
ELEMENTS
ENZYMES
FUNCTIONS
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
METALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PHAGOCYTES
RODENTS
SACCHARIDES
SOMATIC CELLS
TRANSITION ELEMENTS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550201 - Biochemistry- Tracer Techniques