Radiometric method for the rapid detection of Leptospira organisms

Journal Article · Sat Feb 01 00:00:00 EST 1986 · J. Clin. Microbiol.; (United States)

OSTI ID:5488779

Manca, N; Verardi, R; Colombrita, D; Ravizzola, G; Savoldi, E; Turano, A

A rapid and sensitive radiometric method for detection of Leptospira interrogans serovar pomona and Leptospira interrogans serovar copenhageni is described. Stuart's medium and Middlebrook TB (12A) medium supplemented with bovine serum albumin, catalase, and casein hydrolysate and labeled with /sup 14/C-fatty acids were used. The radioactivity was measured in a BACTEC 460. With this system, Leptospira organisms were detected in human blood in 2 to 5 days, a notably shorter time period than that required for the majority of detection techniques.

• Research Organization: Univ. of Brescia, Italy
• OSTI ID: 5488779
• Journal Information: J. Clin. Microbiol.; (United States), Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BACTERIA
RADIOMETRIC ANALYSIS
SEPTICEMIA
DIAGNOSIS
BLOOD
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CATTLE
MAN
TRACER TECHNIQUES
ANIMALS
BIOLOGICAL MATERIALS
BODY FLUIDS
CHEMICAL ANALYSIS
DOMESTIC ANIMALS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
QUANTITATIVE CHEMICAL ANALYSIS
RUMINANTS
VERTEBRATES
550901* - Pathology- Tracer Techniques