Ouabain sensitivity is linked to ras -transformation in human HOS cells

Journal Article · Tue Apr 29 00:00:00 EDT 1986 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(86)90512-7· OSTI ID:5488185

Benade, L E; Talbot, N; Tagliaferri, P; Hardy, C; Card, J; Noda, M; Najam, N; Bassin, R H

Mouse cells transformed by the retroviral oncogene v-Ki- ras are significantly more sensitive to the toxic effects of 1mM ouabain than are their nontransformed counterparts. We have extended these findings to a human cell line (HOS). HOS cells (ATCC CRL 1543) are relatively resistant to treatment with 1 microM ouabain while KHOS cells (transformed by Kirsten murine sarcoma virus) are extremely sensitive. Two flat revertant cell lines isolated from the KHOS line and lacking the v- ras gene sequences are resistant to ouabain. This effect may be observed morphologically and can also be demonstrated by dye exclusion and plating efficiency tests. In addition, the toxic effects of ouabain may be rapidly and efficiently quantitated in a /sup 51/Cr-release assay. This differential lethality may be used to enrich the proportion of non-transformed revertants in populations of mutagen-treated transformed cells.

• Research Organization: American Type Culture Collection, Rockville, MD
• OSTI ID: 5488185
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ONCOGENIC TRANSFORMATIONS
SENSITIVITY
OUABAIN
TOXICITY
CHROMIUM 51
MAN
MICE
NEOPLASMS
REVERTANTS
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL TRANSFORMATIONS
CHROMIUM ISOTOPES
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GLYCOSIDES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MUTANTS
NUCLEI
ORGANIC COMPOUNDS
PRIMATES
RADIOISOTOPES
RODENTS
STROPHANTHINS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
550901 - Pathology- Tracer Techniques