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Title: Comparison of various assays to quantitate macrophage activation by biological response modifiers

Abstract

Macrophages treated with various compounds that enhance host antitumor resistance exhibit measurable changes in metabolism, function, and surface antigens. In this study, murine peptone-induced peritoneal macrophages were stimulated in vitro by bacterial lipopolysaccharide (LPS), muramyl dipeptide (MDP), and poly I.poly C. They were subsequently compared in their ability to release superoxide and act as tumoristatic and tumoricidal effector cells. Superoxide generation was assayed by the reduction of ferricytochrome C. All three compounds failed to induce significant O/sub 2/- release, unless the cells were also treated with phorbol myristate acetate (PMA). MDP was most active in potentiating the PMA response. In the tumor growth inhibition assay, cytostatic activity was comparable for all three compounds and did not exceed 32 percent. The combination of subthreshold levels of these compounds and hybridoma-derived MAF acted synergistically to induce potent cytostatic activity. In the chromium release assay, LPS and poly I.poly C rendered macrophages cytolytic for P815 target cells at concentrations greater than or equal to 1 microgram/ml. In contrast, significant cytolysis was observed with MDP only at 100 micrograms/ml. Defining precisely the effect of various biological response modifiers on several parameters of macrophage function may facilitate use of these agents in cancer therapy.

Authors:
; ;
Publication Date:
Research Org.:
Lilly Research Labs., Indianapolis, IN
OSTI Identifier:
5483582
Resource Type:
Journal Article
Journal Name:
J. Immunolopharmacol.; (United States)
Additional Journal Information:
Journal Volume: 6:4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; MACROPHAGES; BIOLOGICAL FUNCTIONS; METABOLIC ACTIVATION; ANTIGEN-ANTIBODY REACTIONS; CHROMIUM ISOTOPES; EXPERIMENTAL NEOPLASMS; HYBRIDOMAS; LIPOPOLYSACCHARIDES; MALES; MICE; PHORBOL ESTERS; TRACER TECHNIQUES; ANIMAL CELLS; ANIMALS; CARBOHYDRATES; CARCINOGENS; CONNECTIVE TISSUE CELLS; ESTERS; FUNCTIONS; ISOTOPE APPLICATIONS; ISOTOPES; LIPIDS; MAMMALS; ORGANIC COMPOUNDS; PHAGOCYTES; POLYSACCHARIDES; RODENTS; SACCHARIDES; SOMATIC CELLS; VERTEBRATES; 550301* - Cytology- Tracer Techniques

Citation Formats

Schultz, R M, Nanda, S, and Altom, M G. Comparison of various assays to quantitate macrophage activation by biological response modifiers. United States: N. p., 1984. Web. doi:10.3109/08923978409028603.
Schultz, R M, Nanda, S, & Altom, M G. Comparison of various assays to quantitate macrophage activation by biological response modifiers. United States. https://doi.org/10.3109/08923978409028603
Schultz, R M, Nanda, S, and Altom, M G. 1984. "Comparison of various assays to quantitate macrophage activation by biological response modifiers". United States. https://doi.org/10.3109/08923978409028603.
@article{osti_5483582,
title = {Comparison of various assays to quantitate macrophage activation by biological response modifiers},
author = {Schultz, R M and Nanda, S and Altom, M G},
abstractNote = {Macrophages treated with various compounds that enhance host antitumor resistance exhibit measurable changes in metabolism, function, and surface antigens. In this study, murine peptone-induced peritoneal macrophages were stimulated in vitro by bacterial lipopolysaccharide (LPS), muramyl dipeptide (MDP), and poly I.poly C. They were subsequently compared in their ability to release superoxide and act as tumoristatic and tumoricidal effector cells. Superoxide generation was assayed by the reduction of ferricytochrome C. All three compounds failed to induce significant O/sub 2/- release, unless the cells were also treated with phorbol myristate acetate (PMA). MDP was most active in potentiating the PMA response. In the tumor growth inhibition assay, cytostatic activity was comparable for all three compounds and did not exceed 32 percent. The combination of subthreshold levels of these compounds and hybridoma-derived MAF acted synergistically to induce potent cytostatic activity. In the chromium release assay, LPS and poly I.poly C rendered macrophages cytolytic for P815 target cells at concentrations greater than or equal to 1 microgram/ml. In contrast, significant cytolysis was observed with MDP only at 100 micrograms/ml. Defining precisely the effect of various biological response modifiers on several parameters of macrophage function may facilitate use of these agents in cancer therapy.},
doi = {10.3109/08923978409028603},
url = {https://www.osti.gov/biblio/5483582}, journal = {J. Immunolopharmacol.; (United States)},
number = ,
volume = 6:4,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 1984},
month = {Sun Jan 01 00:00:00 EST 1984}
}