Conversions of excision-repairable DNA lesions to micronuclei within one cell cycle in human lymphocytes
- CSIRO, Adelaide (Australia)
The human lymphocyte micronucleus (MN) assay is relatively insensitive to genotoxic agents that predominantly induce excision-repairable lesions such as adducts and abasic sites. In this study the authors have explored the possibility of using cytosine arabinoside (ARA) to convert excision-repairable DNA lesions to micronuclei (MN) within one cell cycle. The system consisted of human lymphocytes as target cells, the cytokinesis-block (CB) method for identifying cells that had completed one nuclear division only, and X-rays, methylnitrosourea (MNU), and ultraviolet light (UV) as mutagens. With each mutagen they have observed significant increments induced MN in the cultures that had also been treated with ARA during G{sub 1}. These observations suggested that the combined ARA and cytokinesis-block micronucleus (CBMN) method may enhance the detection of exposure to genotoxic agents that predominantly induce excision-repairable lesions.
- OSTI ID:
- 5481736
- Journal Information:
- Environmental and Molecular Mutagenesis; (United States), Vol. 19:1; ISSN 0893-6692
- Country of Publication:
- United States
- Language:
- English
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DNA
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EXTREME ULTRAVIOLET RADIATION
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ALDEHYDES
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ELECTROMAGNETIC RADIATION
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MUTAGENS
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560300* - Chemicals Metabolism & Toxicology