Biodistribution of an anti-interleukin 2 receptor monoclonal antibody in rat recipients of a heart allograft, and its use as a rejection marker in gamma scintigraphy

Journal Article · Fri Sep 01 00:00:00 EDT 1989 · Transplantation; (USA)

DOI:https://doi.org/10.1097/00007890-198909000-00002· OSTI ID:5480491

Thedrez, P; Paineau, J; Jacques, Y; Chatal, J F; Pelegrin, A; Bouchaud, C; Soulillou, J P ^[1]

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1. Institut National Pour la Recherche Scientifique et Medicale U.211 (France)

Anti-interleukin-2 receptor monoclonal antibodies have been shown to prevent allograft rejection. This paper reports on the biodistribution of a mouse MoAb directed at the 55 Kd alpha chain of rat interleukin-2 receptor (IL2-R) during allograft rejection. Only a low percentage (approximately 1%) of intact 125I-labeled MoAb was detected in the rejected graft, and irrelevant control IgG1 was found at a similar level. This suggests that most of the injected intact MoAb bound to graft tissue via its monomorphic Fc segment. In contrast, OX39 F(ab')2 fragments showed a preferential localization in the rejected allograft and did not bind to the LEW-to-LEW syngeneic heart graft. Irrelevant F(ab')2 did not concentrate in the allogeneic graft. Accordingly, F(ab')2 fragments from OX39 or irrelevant MoAb were used for gamma-scintigraphy on allograft recipients together with biodistribution studies. Results show that scintigraphy was able to detect allograft accumulation of 131I OX39 F(ab')2, whereas no imaging was obtained when OX39 F(ab')2 was used in the syngeneic combination or when irrelevant 131-IgG1 F(ab')2 was given to allograft recipients. This method, applied to the clinical situation, could be of interest for detection of early graft rejection episodes by immunoscintigraphy using reagents specific for activation determinants on lymphocyte membranes, such as anti-interleukin-2 receptor MoAb.

• OSTI ID: 5480491
• Journal Information: Transplantation; (USA), Vol. 48:3; ISSN 0041-1337
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
GRAFT-HOST REACTION
DIAGNOSIS
LYMPHOKINES
RECEPTORS
MONOCLONAL ANTIBODIES
TISSUE DISTRIBUTION
HEART
IMMUNOGLOBULINS
IODINE 125
IODINE 131
LYMPHOCYTES
RADIOIMMUNOSCINTIGRAPHY
RATS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARDIOVASCULAR SYSTEM
CONNECTIVE TISSUE CELLS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
GROWTH FACTORS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOIMMUNODETECTION
RADIOISOTOPE SCANNING
RADIOISOTOPES
RODENTS
SCINTISCANNING
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics