In vivo biosynthesis of L-(/sup 35/S)Cys-arginine vasopressin, -oxytocin, and -somatostatin: rapid estimation using reversed phase high pressure liquid chromatography. [Rats]

Journal Article · Thu Jan 01 00:00:00 EST 1981 · Endocrinology; (United States)

OSTI ID:5479358

Franco-Bourland, R E; Fernstrom, J D

L(/sup 35/S)Cys-arginine vasopressin, -oxytocin, and -somatostatin were purified from hypothalami and neurohypophyses 4 h after rats received L(/sup 35/S)Cys via the third ventricle. After acetic acid extraction, Sephadex G-25 filtration, and chemoadsorption to C18-silica (Sep-Pak cartridges), the labeled peptides were rapidly separated by gradient elution, reversed phase, high pressure liquid chromatography (HPLC). The identity and isotopic purity of the labeled peptides were determined by several reversed phase HPLC procedures in conjunction with chemical modification. The labeled peptide fractions were at least 50% radiochemically pure. Using this HPLC isolation procedure, incorporation of L-(/sup 35/S)Cys into each peptide was determined in hydrated and dehydrated rats. Label incorporation into arginine vasopressin and oxytocin in the hypothalamus and the neurohypophysis of dehydrated rats was 2-3 times greater than that in hydrated rats. Incorporation of label into hypothalamic and neurohypophyseal somatostatin was unaffected by the hydration state of the animal. This procedure thus provides a very rapid, but sensitive, set of techniques for studying the control of small peptide biosynthesis in the brain.

• Research Organization: Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge
• OSTI ID: 5479358
• Journal Information: Endocrinology; (United States), Vol. 109:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
OXYTOCIN
BIOSYNTHESIS
SOMATOSTATIN
VASOPRESSIN
BRAIN
IN VIVO
RATS
SULFUR 35
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CENTRAL NERVOUS SYSTEM
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
HORMONES
ISOTOPES
LIGHT NUCLEI
MAMMALS
NERVOUS SYSTEM
NUCLEI
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOISOTOPES
RODENTS
SEPARATION PROCESSES
SULFUR ISOTOPES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
560172 - Radiation Effects- Nuclide Kinetics & Toxicology- Animals- (-1987)