Multipurpose ligand, DAKLI (Dynorphin A-analogue Kappa LIgand), with high affinity and selectivity for dynorphin (. kappa. opioid) binding sites
Journal Article
·
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
- Stanford Univ., CA (USA)
The authors describe a synthetic ligand, DALKI (Dynorphin A-analogue Kappa LIgand), related to the opioid peptide dynorphin A. A single reactive amino group at the extended carboxyl terminus permits various reporter groups to be attached, such as {sup 125}I-labeled Bolton-Hunter reagent, fluorescein isothiocyanate, or biotin. These derivatives have high affinity and selectivity for the dynorphin ({kappa} opioid) receptor. An incidental finding is that untreated guinea pig brain membranes have saturable avidin binding sites.
- OSTI ID:
- 5478270
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 85:19; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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OSTI ID:5478270
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
LABELLED COMPOUNDS
CHEMICAL PREPARATION
LIGANDS
BIOSYNTHESIS
NARCOTICS
RECEPTORS
BIOCHEMICAL REACTION KINETICS
BRAIN
CELL MEMBRANES
GUINEA PIGS
IODINATION
IODINE 125
ISOTHERMS
LIQUID COLUMN CHROMATOGRAPHY
PEPTIDES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HALOGENATION
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SEPARATION PROCESSES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
LABELLED COMPOUNDS
CHEMICAL PREPARATION
LIGANDS
BIOSYNTHESIS
NARCOTICS
RECEPTORS
BIOCHEMICAL REACTION KINETICS
BRAIN
CELL MEMBRANES
GUINEA PIGS
IODINATION
IODINE 125
ISOTHERMS
LIQUID COLUMN CHROMATOGRAPHY
PEPTIDES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HALOGENATION
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SEPARATION PROCESSES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques