The effect of interferon on the receptor sites to rabies virus on mouse neuroblastoma cells
The binding of rabies virus to mouse neuroblastoma cells (MNA) primed with alpha interferon (IFN-{alpha}), beta interferon (IFN-{beta}), or alpha bungarotoxin (BTX) was examined. A saturable number of receptor sites to rabies virus was calculated by increasing the amount of {sup 3}H-CVS added to a constant number of untreated MNA cells. MNA cells were then exposed to 20 I.U. of IFN-{alpha}, IFN-{beta}, or 1 {mu}g of BTX and assayed to determine if these treatments had an effect on the number of receptor sites to rabies virus. Total amount of {sup 3}H-CVS bound to MNA cells was determined during a three hour incubation period. Cold competition assays using 1,000 fold excess unlabeled CVS were used to determine non-specific binding for each treatment. Specific binding was then calculated by subtracting non-specific binding from the total amount of CVS bound to MNA cells. A similar amount of total viral protein bound to untreated and IFN-{beta}, and BTX treated cells after 180 minutes of incubation. The bound protein varied by only 0.07 {mu}g. However, the amount of specific and non-specific binding varied a great deal between treatments. BTX caused an increase in non-specific and a decrease in specific binding of rabies virus. IFN-{beta} produced variable results in non-specific and specific binding while IFN-{alpha} caused mainly specific binding to occur. The most significant change brought about by IFN-{alpha} was an increase in the rate of viral attachment. At 30 minutes post-infection, IFN-{alpha} treated cells had bound 90% of the total amount of virus bound to untreated cells after 180 minutes. The increased binding rate did not cause a productive infection of rabies virus. No viral production was evident after an incubation period of 48 hours in either IFN-{alpha} or IFN-{beta} treated cells.
- Research Organization:
- Kansas State Univ., Manhattan, KS (United States)
- OSTI ID:
- 5473898
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
INTERFERON
BIOLOGICAL EFFECTS
RABIES
INFECTIVITY
VIRUSES
RECEPTORS
BIOCHEMICAL REACTION KINETICS
MICE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
ANIMAL CELLS
ANIMALS
DISEASES
GROWTH FACTORS
HYDROGEN COMPOUNDS
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
KINETICS
LYMPHOKINES
MAMMALS
MEMBRANE PROTEINS
MICROORGANISMS
MITOGENS
NERVOUS SYSTEM DISEASES
ORGANIC COMPOUNDS
PARASITES
PROTEINS
REACTION KINETICS
RODENTS
VERTEBRATES
VIRAL DISEASES
550901* - Pathology- Tracer Techniques