Primary structure of the human follistatin precursor and its genomic organization

Journal Article · Wed Jun 01 00:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.12.4218· OSTI ID:5472951

Shimasaki, Shunichi; Koga, Makoto; Esch, F; Cooksey, K; Mercado, M; Koba, A; Ueno, Naoto; Ying, Shaoyao; Ling, N; Guillemin, R ^[1]

---

1. Salk Institute, La Jolla, CA (USA)

Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. By use of the recently characterized porcine follistatin cDNA as a probe to screen a human testis cDNA library and a genomic library, the structure of the complete human follistatin precursor as well as its genomic organization have been determined. Three of eight cDNA clones that were sequenced predicted a precursor with 344 amino acids, whereas the remaining five cDNA clones encoded a 317 amino acid precursor, resulting from alternative splicing of the precursor mRNA. Mature follistatins contain four contiguous domains that are encoded by precisely separated exons; three of the domains are highly similar to each other, as well as to human epidermal growth factor and human pancreatic secretory trypsin inhibitor. The genomic organization of the human follistatin is similar to that of the human epidermal growth factor gene and thus supports the notion of exon shuffling during evolution.

• OSTI ID: 5472951
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 85:12; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FSH
INHIBITION
PROTEINS
AMINO ACID SEQUENCE
RECOMBINANT DNA
DNA SEQUENCING
BIOLOGICAL EVOLUTION
EPIDERMIS
GROWTH FACTORS
HYBRIDIZATION
MEN
MESSENGER-RNA
PHOSPHORUS 32
TESTES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DNA
EPITHELIUM
GONADOTROPINS
GONADS
HORMONES
ISOTOPES
LIGHT NUCLEI
MALE GENITALS
MALES
MAMMALS
MAN
MITOGENS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHOSPHORUS ISOTOPES
PITUITARY HORMONES
PRIMATES
RADIOISOTOPES
RNA
SKIN
STRUCTURAL CHEMICAL ANALYSIS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques