Diode-laser-based optical rotation detector for high-performance liquid chromatography and on-line polarimetric analysis

Journal Article · Thu Jun 01 00:00:00 EDT 1989 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00186a013· OSTI ID:5468198

Lloyd, D K; Goodall, D M; Scrivener, H ^[1]

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1. Univ. of York, Heslington (England)

A simple polarimeter with microdegree sensitivity based on a near-infrared (820 nm) semiconductor diode laser is described, and signal to noise ratio analysis is discussed. With the use of small-volume flow cells (typically 8 {mu}L) its utility as a high-performance liquid chromatography (HPLC) detector in the gradient elution separation of carbohydrates is shown. Coupled with a UV detector, the HPLC enantiomeric purity determination of D-/L-tryptophan mixtures without chiral separation is demonstrated. On a laboratory-scale system an application from the sugar industry is reported, monitoring molasses flow streams which are opaque at visible wave-lengths.

• OSTI ID: 5468198
• Journal Information: Analytical Chemistry (Washington); (United States), Vol. 61:11; ISSN 0003-2700
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
CARBOHYDRATES
LIQUID COLUMN CHROMATOGRAPHY
ENANTIOMORPHS
MEASURING INSTRUMENTS
MEASURING METHODS
POLARIMETRY
SEMICONDUCTOR LASERS
CHROMATOGRAPHY
LASERS
ORGANIC COMPOUNDS
SEMICONDUCTOR DEVICES
SEPARATION PROCESSES
SOLID STATE LASERS
400102* - Chemical & Spectral Procedures