Interconversion of high density lipoproteins during incubation of human plasma

Journal Article · Fri May 15 00:00:00 EDT 1981 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/S0006-291X(81)80109-X· OSTI ID:5467629

Nichols, A V; Gong, E L; Blanche, P J

Incubation (6h,37/sup 0/) of untreated human plasma resulted in marked reductions in concentrations of two major subpopulations (mean sizes: approx.84 angstroms and approx.79 angstroms) of the HDL/sub 3/ subclass. These reductions were accompanied by the appearance of large species with mean particle size (95.2 angstroms) in the range of the HDL/sub 2a/ subpopulations. In the presence of mercaptoethanol (14 mM), incubation resulted in further reduction of the two HDL/sub 3/ subpopulations but now with formation of both larger (95.4 angstroms) and smaller (77.2 and 75.2 angstroms) species. Inhibitors of lecithin:cholesterol acyltransferase showed varying effectivenss in modifying the extent and character of the interconversion. With paraoxon (2 mM) the interconversion was much reduced and was associated with the formation predominantly of smaller particles (74.6 angstroms); with 5,5'-dithiobis-(2-nitrobenzoic acid) (1.4 mM) the interconversion resembled that produced with mecaptoethanol but to a considerably lesser degree. Incubation of HDL, ultracentrifugally isolated from nonincubated plasma containing paraoxon, resulted in negligible interconversion, suggesting that other plasma components participate in the process.

• Research Organization: Lawrence Berkeley Lab., CA
• OSTI ID: 5467629
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 100:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BLOOD PLASMA
INCUBATION
LIPOPROTEINS
CHEMICAL REACTIONS
ELECTROPHORESIS
ENZYME INHIBITORS
MEDIUM TEMPERATURE
PATIENTS
RESPONSE MODIFYING FACTORS
ULTRACENTRIFUGATION
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CENTRIFUGATION
LIPIDS
MATERIALS
ORGANIC COMPOUNDS
PROTEINS
SEPARATION PROCESSES
550200* - Biochemistry