Synergism of cellulases from Trichoderma reesei in the degradation of cellulose
The action of cellobiohydrolases I and II (CBHI and CBHII) and endoglucanases I and II (EGI and EGII) purified from Trichoderma reesei was evaluated against various substrates. CBHI degraded the ..beta..-D-glucan from barley in a typical endo pattern. With cellulose substrates, the synergism between CBHI and endoglucanase I or II depended on the structural and ultrastructural features of the substrate. This effect, unrelated to endo-exo cooperation, was found with substrates of intermediate crystallinity whereas weak or no synergism was recorded with cellulose microcrystals or the soluble carboxy-methyl cellulose derivative. Synergistic degradation of cellulose was also recorded with mixtures of CBHI and CBHII. On the other hand, synergism between endoglucanases and CBHII followed the pattern expected for an endo-exo cooperation. These results presented support evidence for multiple types of cooperation between the cellulolytic enzymes. 30 references, 7 figures, 1 table.
- Research Organization:
- Scientific and Medical Univ., Grenoble, Sant-Martin-d'Heres, France
- OSTI ID:
- 5465674
- Journal Information:
- Bio/Technology; (United States), Vol. 3:8
- Country of Publication:
- United States
- Language:
- English
Similar Records
Engineering cellulase mixtures by varying the mole fraction of Thermomonospora fusca E[sub 5] and E[sub 3], Trichoderma reesei CBHI, and Caldocellum saccharolyticum [beta]-glucosidase
Specific quantification of Trichoderma reesei cellulases in reconstituted mixtures and its application to cellulase-cellulose binding studies
Related Subjects
CELLULASE
CELLULOLYTIC ACTIVITY
SYNERGISM
CELLULOSE
ENZYMATIC HYDROLYSIS
EXPERIMENTAL DATA
MIXTURES
SUBSTRATES
TRICHODERMA VIRIDE
CARBOHYDRATES
CHEMICAL REACTIONS
DATA
DECOMPOSITION
DISPERSIONS
ENZYME ACTIVITY
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
INFORMATION
LYSIS
NUMERICAL DATA
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
TRICHODERMA
090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)
140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)