Inducible error-prone repair in B. subtilis. Progress report, May 1, 1983-April 30, 1984

Technical Report · Thu Dec 01 00:00:00 EST 1983

OSTI ID:5465236

Yasbin, R E

DNA repair mechanisms in Bacillus subtilis were investigated following mutagenesis via ultraviolet radiation or by chemical mutagens. A bioassay is described whereby the efficiency of repair mechanisms can be measured. DNA cloning studies to transfer the photoreactivation gene from E. coli to B. subtilis are reported. The mutation, which induces the SOS-like system in B. subtilis when grown at 45/sup 0/C, was characterized in order to begin delineation of the genes controlling this system, efforts directed at isolation and cloning of a DNA Polymerase III gene of B. subtilis are related. (DT)

• Research Organization: Rochester Univ., NY (USA). Dept. of Microbiology
• DOE Contract Number: AC02-81ER60017
• OSTI ID: 5465236
• Report Number(s): DOE/ER/60017-2; ON: DE84004895
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BACILLUS SUBTILIS
DNA-CLONING
MUTANTS
BIOLOGICAL REPAIR
BIOCHEMISTRY
DNA
BIOLOGICAL RADIATION EFFECTS
DNA POLYMERASES
GENES
EMS
BIOLOGICAL EFFECTS
POLYCYCLIC NITRO COMPOUNDS
ESCHERICHIA COLI
NUCLEASES
PLASMIDS
ULTRAVIOLET RADIATION
BACILLUS
BACTERIA
BIOLOGICAL RECOVERY
CELL CONSTITUENTS
CHEMISTRY
CLONING
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
ESTERS
HYDROLASES
MICROORGANISMS
MUTAGENS
NITRO COMPOUNDS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHODIESTERASES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SULFONIC ACID ESTERS
TRANSFERASES
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
560302 - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)
550200 - Biochemistry