Sertoli cells in culture secrete paracrine factor(s) that inhibit peritubular myoid cell proliferation: identification of heparinoids as likely candidates

Journal Article · Sat Jun 01 00:00:00 EDT 1991 · Journal of Cellular Physiology; (United States)

DOI:https://doi.org/10.1002/jcp.1041470313· OSTI ID:5464095

Tung, P S; Fritz, I B ^[1]

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1. Banting and Best Department of Medical Research, University of Toronto, Ontario (Canada)

Conditioned medium from Sertoli cells, prepared from testes of 20-day-old rats, contains component(s) that inhibit the incorporation of (3H)-thymidine into DNA of peritubular myoid cells (PMC) and inhibit the proliferation of PMC. These components are trypsin-resistant, heat-stable compounds having a molecular weight less than 30,000. The active inhibitory components in Sertoli cell conditioned medium are inactivated by treatment with heparinase, but not by treatment with hyaluronidase or chondroitin sulfate lyases. Addition of heparin or heparan sulfate results in inhibition of DNA synthesis by PMC in a dose-dependent manner, whereas other glycosaminoglycans (GAGs) examined (hyaluronic acid, keratan sulfate, and chondroitin sulfate) have no detectable effects. Heparin and heparan sulfate are unique among GAGs tested in inhibiting the characteristic multilayer growth pattern of PMC following the attainment of confluence in serum-rich medium. On the basis of these and other data presented, it is concluded that heparin and other heparin-like GAGs synthesized by Sertoli cells are implicated in the modulation of growth of PMC in vitro during co-culture. It is postulated that heparin may play a similar role in maintaining the quiescent peritubular myoid cell phenotype in vivo.

• OSTI ID: 5464095
• Journal Information: Journal of Cellular Physiology; (United States), Vol. 147:3; ISSN 0021-9541
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
CELL PROLIFERATION
HEPARIN
BIOCHEMICAL REACTION KINETICS
CELL CULTURES
DNA
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
GLYCOPROTEINS
INHIBITION
MICE
MITOSIS
MOLECULAR WEIGHT
PHENOTYPE
RATS
TESTES
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
ANIMALS
ANTICOAGULANTS
AZINES
BODY
CARBOHYDRATES
CELL DIVISION
DRUGS
GONADS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
MALE GENITALS
MAMMALS
MUCOPOLYSACCHARIDES
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
POLYSACCHARIDES
PROTEINS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
RODENTS
SACCHARIDES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques