Accurate measurement of intestinal transit in the rat
A new method for quantifying intestinal transit was evaluated by comparison with two other popular techniques. The distribution of radiochromium (51Cr) throughout the small intestine of rats previously treated with saline (1.0 ml/kg s.c.), capsaicin (10 mg/kg s.c.), hexamethonium (20 mg/kg i.p.), D-ala2-met-enkephalinamide (1.0 microgram i.c.v.), or neostigmine (0.1 mg/kg i.p.) was quantified by (1) measuring the most distal intestinal segment reached by chromium, (2) calculating the slope produced by linear regression analysis on cumulative percent chromium that had passed through each segment, and (3) determining the geometric center of the distribution of chromium throughout the small intestine. It was concluded that the geometric center methods for quantifying intestinal transit provides the most sensitive and reliable measure of intestinal transit. Less sensitive techniques often fail to detect important effects of drugs on intestinal transit.
- Research Organization:
- Department of Pharmacology, Arizona Health Sciences Center, Tucson, AZ
- OSTI ID:
- 5462575
- Journal Information:
- J. Pharmacol. Methods; (United States), Vol. 6:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHROMIUM 51
TISSUE DISTRIBUTION
SMALL INTESTINE
RADIONUCLIDE KINETICS
AMIDES
RATS
REGRESSION ANALYSIS
SALTS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CHROMIUM ISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GASTROINTESTINAL TRACT
INTERMEDIATE MASS NUCLEI
INTESTINES
ISOTOPES
MAMMALS
MATHEMATICS
NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RADIOISOTOPES
RODENTS
STATISTICS
VERTEBRATES
560172* - Radiation Effects- Nuclide Kinetics & Toxicology- Animals- (-1987)