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Title: Aortic lipid and /sup 125/I-albumin accumulation in streptozotocin-diabetic guinea pigs: prevention by insulin treatment

Abstract

Diabetes mellitus, a major risk factor of atherosclerosis, is associated with the aortic accumulation of macromolecules. The authors have examined this relationship in the streptozotocin (STZ)-diabetic guinea pig, a species (like man) unable to synthesize ascorbic acid and susceptible to atherosclerosis. Male Dunkin-Hartley guinea pigs received STZ (150 mg/kg, i.c.) or vehicle (control). After 5 days, insulin (10 U/kg/day) was given to half the STZ animals (STZ-INS0 while the remaining half (STZ-SAL) and controls received saline. 25 days later, animals were given /sup 125/I-albumin (100 ..mu..Ci/kg, i.a.). Activity was determined in plasma at 5 (C/sub p5), 15 and 30 minutes, and in the upper thoracic aorta after 30 minutes. Histopathological changes were evaluated in the lower aorta. Aortic albumin permeability defined as cpm/cm/sup 2//sec, cpm/cm/sup 2//sec/C/sub p5/, or cpm/C/sub p5//g tissue was significantly elevated in the STZ-SAL group compared to both STZ-INS and control groups; these latter two groups were not significantly different from each other. Oil-Red-O positive material (lipid) occurred at multifocal areas within the intima of the STZ-SAL animals only. This study demonstrates (1) an abnormal increase in aortic permeability to albumin, (2) histological evidence of early atherosclerotic lesions, and (3) that insulin treatment can prevent these angiopathiesmore » in this STZ-diabetic animal model.« less

Authors:
; ;
Publication Date:
Research Org.:
Univ. of Mississippi, University
OSTI Identifier:
5459367
Report Number(s):
CONF-8604222-
Journal ID: CODEN: FEPRA; TRN: 86-028528
Resource Type:
Conference
Journal Name:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
Additional Journal Information:
Journal Volume: 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ALBUMINS; BIOLOGICAL ACCUMULATION; AORTA; PERMEABILITY; DIABETES MELLITUS; PATHOGENESIS; LIPIDS; BIOLOGICAL MODELS; GUINEA PIGS; INSULIN; IODINE 125; PATHOLOGICAL CHANGES; TRACER TECHNIQUES; ANIMALS; ARTERIES; BETA DECAY RADIOISOTOPES; BLOOD VESSELS; BODY; CARDIOVASCULAR SYSTEM; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; ENDOCRINE DISEASES; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; METABOLIC DISEASES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; PROTEINS; RADIOISOTOPES; RODENTS; VERTEBRATES; 550901* - Pathology- Tracer Techniques

Citation Formats

Schlosser, M J, Bannon, A W, and Verlangieri, A J. Aortic lipid and /sup 125/I-albumin accumulation in streptozotocin-diabetic guinea pigs: prevention by insulin treatment. United States: N. p., 1986. Web.
Schlosser, M J, Bannon, A W, & Verlangieri, A J. Aortic lipid and /sup 125/I-albumin accumulation in streptozotocin-diabetic guinea pigs: prevention by insulin treatment. United States.
Schlosser, M J, Bannon, A W, and Verlangieri, A J. 1986. "Aortic lipid and /sup 125/I-albumin accumulation in streptozotocin-diabetic guinea pigs: prevention by insulin treatment". United States.
@article{osti_5459367,
title = {Aortic lipid and /sup 125/I-albumin accumulation in streptozotocin-diabetic guinea pigs: prevention by insulin treatment},
author = {Schlosser, M J and Bannon, A W and Verlangieri, A J},
abstractNote = {Diabetes mellitus, a major risk factor of atherosclerosis, is associated with the aortic accumulation of macromolecules. The authors have examined this relationship in the streptozotocin (STZ)-diabetic guinea pig, a species (like man) unable to synthesize ascorbic acid and susceptible to atherosclerosis. Male Dunkin-Hartley guinea pigs received STZ (150 mg/kg, i.c.) or vehicle (control). After 5 days, insulin (10 U/kg/day) was given to half the STZ animals (STZ-INS0 while the remaining half (STZ-SAL) and controls received saline. 25 days later, animals were given /sup 125/I-albumin (100 ..mu..Ci/kg, i.a.). Activity was determined in plasma at 5 (C/sub p5), 15 and 30 minutes, and in the upper thoracic aorta after 30 minutes. Histopathological changes were evaluated in the lower aorta. Aortic albumin permeability defined as cpm/cm/sup 2//sec, cpm/cm/sup 2//sec/C/sub p5/, or cpm/C/sub p5//g tissue was significantly elevated in the STZ-SAL group compared to both STZ-INS and control groups; these latter two groups were not significantly different from each other. Oil-Red-O positive material (lipid) occurred at multifocal areas within the intima of the STZ-SAL animals only. This study demonstrates (1) an abnormal increase in aortic permeability to albumin, (2) histological evidence of early atherosclerotic lesions, and (3) that insulin treatment can prevent these angiopathies in this STZ-diabetic animal model.},
doi = {},
url = {https://www.osti.gov/biblio/5459367}, journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 45:3,
place = {United States},
year = {Sat Mar 01 00:00:00 EST 1986},
month = {Sat Mar 01 00:00:00 EST 1986}
}

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