Tuberoinfundibular dopaminergic neurons of the hypothalamus are progestin target cells

Conference · Sat Mar 01 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5453884

Sar, M

To find out a direct relationship between progestin target neurons and tuberoinfundibular dopaminergic neurons colocalization of /sup 3/H ORG 2058 (a synthetic progestin) and tyrosine hydroxylase, TH, antibodies were studied by combined autoradiography and immunohistochemistry. Eight 23 day-old ovariectomized and adrenalectomized rats were injected s.c. 17-beta estradiol, daily for 4 days. On the 5th day each animal was injected i.v. 1.0 ug per 100g b.w. of /sup 3/H ORG 2058. Two animals each received 1mg of unlabeled ORG 2058 15 min prior to the injection of /sup 3/H ORG 2058 to show the specificity of localization. Animals were sacrificed after 15 or 30 min, brains were dissected, frozen and processed for autoradiography. The autoradiograms were stained immunohistochemically with antibodies to TH. TH-containing cells in the arcuate nucleus and in the hypothalamic periventricular nucleus (Group A12) showed concentration of radioactivity in their nuclei, while TH cells in Group A11, A13, A14, and in the substantia nigra (Group A9), and ventral tegmental area (Group A10) did not show nuclear concentration of /sup 3/H ORG 2058. Competition studies with unlabeled ORG 2058 abolished the nuclear uptake of radioactivity in TH containing neurons. The results suggest a direct affect of progestin on tuberoinfundibular dopaminergic neurons.

• Research Organization: Univ. of North Carolina, Chapel Hill
• OSTI ID: 5453884
• Report Number(s): CONF-8604222-; TRN: 86-026574
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HYPOTHALAMUS
AUTORADIOGRAPHY
PROGESTERONE
BIOLOGICAL LOCALIZATION
ANTIBODIES
CELL NUCLEI
DOPAMINE
ESTRADIOL
HYDROXYLASES
NERVE CELLS
RATS
TRITIUM COMPOUNDS
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
BRAIN
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
DRUGS
ENZYMES
ESTRANES
ESTROGENS
HORMONES
HYDROXY COMPOUNDS
KETONES
LABELLED COMPOUNDS
MAMMALS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PHENOLS
POLYPHENOLS
PREGNANES
RODENTS
SOMATIC CELLS
STEROID HORMONES
STEROIDS
SYMPATHOMIMETICS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques