skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Na/sup +/-dependent transport of /sup 14/C-L-lysine across bullfrog alveolar epithelium

Abstract

Transepithelial transport of the basic amino acid L-lysine has been studied utilizing the isolated intact bullfrog lung mounted in the Ussing chamber. Lungs were excised from doubly pithed bullfrogs and sandwiched between two hemichambers. /sup 14/C-(U)-L-lysine was added to the upstream reservoir of amphibian Ringer solution, while the tissue was short-circuited. Two lungs from the same animal were used simultaneously to determine the two opposite unidirectional fluxes. Downstream and upstream radioactivities were assayed and used to estimate the apparent permeability (P) of the labeled lysine. Results indicate that the apparent P of /sup 14/C-L-lysine measured in the alveolar (M) to the pleural (S) direction is 19.06 (+- 2.84) x 10/sup -7/ cm/s and P in the S to M direction is 3.29 (+- 0.02) x 10/sup -7/ cm/s. When the 100 mM NaCl in the bath was replaced by 110 mM choline chloride, the flux of /sup 14/C-L-lysine from the alveolar to the pleural side decreased to the same value as that in the opposite direction. The flux from the pleural to the alveolar direction in the absence of Na/sup +/ did not change. These results suggest that the alveolar epithelium exhibits Na/sup +/-dependent amino acid (L-lysine) transport in themore » M->S, but not in the S->M, direction.« less

Authors:
;
Publication Date:
Research Org.:
Univ. of California, Los Angeles
OSTI Identifier:
5450915
Report Number(s):
CONF-8604222-
Journal ID: CODEN: FEPRA; TRN: 86-028365
Resource Type:
Conference
Journal Name:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
Additional Journal Information:
Journal Volume: 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; EPITHELIUM; PERMEABILITY; LYSINE; MEMBRANE TRANSPORT; CARBON 14 COMPOUNDS; CATIONS; FROGS; LUNGS; SODIUM COMPOUNDS; TRACER TECHNIQUES; ALKALI METAL COMPOUNDS; AMINO ACIDS; AMPHIBIANS; ANIMAL TISSUES; ANIMALS; AQUATIC ORGANISMS; BODY; CARBOXYLIC ACIDS; CHARGED PARTICLES; IONS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; RESPIRATORY SYSTEM; TISSUES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Kim, K J, and Crandall, E D. Na/sup +/-dependent transport of /sup 14/C-L-lysine across bullfrog alveolar epithelium. United States: N. p., 1986. Web.
Kim, K J, & Crandall, E D. Na/sup +/-dependent transport of /sup 14/C-L-lysine across bullfrog alveolar epithelium. United States.
Kim, K J, and Crandall, E D. 1986. "Na/sup +/-dependent transport of /sup 14/C-L-lysine across bullfrog alveolar epithelium". United States.
@article{osti_5450915,
title = {Na/sup +/-dependent transport of /sup 14/C-L-lysine across bullfrog alveolar epithelium},
author = {Kim, K J and Crandall, E D},
abstractNote = {Transepithelial transport of the basic amino acid L-lysine has been studied utilizing the isolated intact bullfrog lung mounted in the Ussing chamber. Lungs were excised from doubly pithed bullfrogs and sandwiched between two hemichambers. /sup 14/C-(U)-L-lysine was added to the upstream reservoir of amphibian Ringer solution, while the tissue was short-circuited. Two lungs from the same animal were used simultaneously to determine the two opposite unidirectional fluxes. Downstream and upstream radioactivities were assayed and used to estimate the apparent permeability (P) of the labeled lysine. Results indicate that the apparent P of /sup 14/C-L-lysine measured in the alveolar (M) to the pleural (S) direction is 19.06 (+- 2.84) x 10/sup -7/ cm/s and P in the S to M direction is 3.29 (+- 0.02) x 10/sup -7/ cm/s. When the 100 mM NaCl in the bath was replaced by 110 mM choline chloride, the flux of /sup 14/C-L-lysine from the alveolar to the pleural side decreased to the same value as that in the opposite direction. The flux from the pleural to the alveolar direction in the absence of Na/sup +/ did not change. These results suggest that the alveolar epithelium exhibits Na/sup +/-dependent amino acid (L-lysine) transport in the M->S, but not in the S->M, direction.},
doi = {},
url = {https://www.osti.gov/biblio/5450915}, journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 45:3,
place = {United States},
year = {Sat Mar 01 00:00:00 EST 1986},
month = {Sat Mar 01 00:00:00 EST 1986}
}

Conference:
Other availability
Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that hold this conference proceeding.

Save / Share: