Interactions between properties of amended strip mine spoils and microbial activities
Properties of strip mine spoils before and after amendment with varying levels of carbon and nitrogen sources are characterized and compared with properties of similarly amended garden soil samples. Changes in spoils as reflected in the stimulation of microbial populations, rate of nitrate formation, the turnover of microbial biomass and the growth yields of white clover and rye grass are evaluated. Limed spoils and garden soils were fertilized and incubated at 25/sup 0/C following amendments with organic substrates. Changes in parameters related to soil fertility status were analyzed on a weekly basis. The possible identity and the toxic effects on white clover and a Rhizobium of acidity factors present in strip mine spoils were evaluated using soil experiments and pure culture studies in artificial culture media. The results indicate that acid spoils contain low numbers of viable microorganisms which readily respond to soil amendment with substrates. No nitrification occurs in acid spoils but liming and inoculation with compost infusion stimulate active nitrification. Aluminum, manganese and acidity appear to be important factors which inhibit the survival of plants and microbes in spoils. Adequate liming improves rhizobial survival and growth and nodulation of white clover in spoils. Acidity factors have greater impact on Rhizobium than on the white clover host under nutritionally independent conditions. Increase in inoculum size enhances nodulation and growth of clover at low aluminum levels. Reducing the time of exposure of rhizobia to acidity factors outside the symbiotic host does not appear to enhance the growth yield of clover under symbiotic conditions. Molds appear to contribute more to the increased aggregate stability observed in amended soils than bacteria and actinomycetes.
- OSTI ID:
- 5447988
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
01 COAL, LIGNITE, AND PEAT
54 ENVIRONMENTAL SCIENCES
ACID MINE DRAINAGE
BIOLOGICAL EFFECTS
CLOVER
PLANT GROWTH
GRASS
MICROORGANISMS
POPULATION DYNAMICS
SPOIL BANKS
REVEGETATION
ALUMINIUM
LAND RECLAMATION
MANGANESE
PH VALUE
SURFACE MINING
ELEMENTS
GROWTH
LEGUMINOSAE
METALS
MINING
PLANTS
TRANSITION ELEMENTS
560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)
560303 - Chemicals Metabolism & Toxicology- Plants- (-1987)
010800 - Coal
Lignite
& Peat- Waste Management
510500 - Environment
Terrestrial- Site Resource & Use Studies- (-1989)