Interferon-gamma-treated murine macrophages inhibit growth of tubercle bacilli via the generation of reactive nitrogen intermediates

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Cellular Immunology; (United States)

DOI:https://doi.org/10.1016/0008-8749(91)90014-3· OSTI ID:5447853

Denis, M ^[1]

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1. Unite de recherche, Centre de pneumologie, Hopital Laval, Sainte-Foy, Quebec (Canada)

Murine peritoneal macrophages were isolated and their ability to restrict growth of a virulent Mycobacterium tuberculosis in response to IFN-gamma was assessed in various conditions. Doses of IFN-gamma ranging from 10 to 100 U stimulated high levels of antimycobacterial activity, as seen by inhibition of growth. Addition of catalase, superoxide dismutase, and other scavengers of reactive oxygen species before infection failed to abrogate this restriction of growth, suggestive of a lack of involvement of reactive oxygen species in this phenomenon. Addition of arginase before infection inhibited the bacteriostatic ability of IFN-gamma-pulsed macrophages as did addition of NG-monomethyl L-arginine, an inhibitor of the synthesis of inorganic nitrogen oxide. In both cases, this inhibition was reversed by adding excess L-arginine in the medium. Moreover, nitrite production in macrophages was correlated with their ability to restrict tubercle bacilli growth. These results imply that nitric oxide or another inorganic nitrogen oxide is an important effector molecule in restricting growth of M. tuberculosis in IFN-gamma-pulsed murine macrophages.

• OSTI ID: 5447853
• Journal Information: Cellular Immunology; (United States), Vol. 132:1; ISSN 0008-8749
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
MYCOBACTERIUM TUBERCULOSIS
GROWTH
NITROGEN OXIDES
BIOLOGICAL EFFECTS
ARGININE
INHIBITION
INTERFERON
MACROPHAGES
MICE
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ELEMENTS
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LYMPHOKINES
MAMMALS
MEMBRANES
MICROORGANISMS
MITOGENS
MYCOBACTERIUM
NITROGEN COMPOUNDS
NONMETALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PHAGOCYTES
PROTEINS
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SEROUS MEMBRANES
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