Quantitative predictability of carcinogenicity of the covalent binding index of chemicals to DNA: Comparison of the in vivo and in vitro assays

Journal Article · Thu Mar 01 00:00:00 EST 1990 · Environmental Health Perspectives; (United States)

DOI:https://doi.org/10.1289/ehp.9084183· OSTI ID:5447591

Taningher, M; Saccomanno, G; Santi, L; Parodi, S ^[1]; Grilli, S ^[2]

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1. Universita di Genova/Istituto Nazionale per la Ricerca sul Cancro (Italy)
2. Universita di Bologna (Italy)

The capability of covalent binding to DNA to predict the initiating potential of chemical carcinogens was compared for the assays performed in vivo (rodent liver DNA) and in vitro (purified DNA incubated in the presence of mouse and rat liver microsomes). A quantitative correlation between DNA adducts and carcinogenic potency was investigated. The in vivo assay appeared slightly, but not significantly, more predictive than the in vitro assay. Also predictivity was slightly higher both in vivo and in vitro when we referred to liver carcinogenicity instead of overall carcinogenicity. The predictive ability found for DNA covalent binding (both in vivo and in vitro) was similar to that of many short-term tests (such as mutagenicity, DNA damage/repair, SCEs, and cell transformation tests). The covalent DNA binding, measured after incubation with DNA in vitro in the presence of liver microsomes, could therefore be a reasonable short-term test offering greater rapidity of execution and requiring the sacrifice of fewer animals than the corresponding in vivo test.

• OSTI ID: 5447591
• Journal Information: Environmental Health Perspectives; (United States), Vol. 84; ISSN 0091-6765
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CARCINOGENESIS
BIOASSAY
CARCINOGENS
DNA ADDUCTS
AFLATOXINS
BENZENE
BENZOPYRENE
CELL TRANSFORMATIONS
CHLORINATED AROMATIC HYDROCARBONS
DNA REPAIR
EMS
IN VITRO
IN VIVO
INDOLES
LIVER
MICE
MICROSOMES
MOLECULAR BIOLOGY
MUTAGENESIS
NITROSO COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
RATS
SISTER CHROMATID EXCHANGES
ADDUCTS
ANIMALS
ANTIGENS
AROMATICS
AZAARENES
AZOLES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CELL CONSTITUENTS
CHROMOSOMAL ABERRATIONS
CONDENSED AROMATICS
DIGESTIVE SYSTEM
ESTERS
GLANDS
HALOGENATED AROMATIC HYDROCARBONS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
MAMMALS
MATERIALS
MUTAGENS
MUTATIONS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PATHOGENESIS
PYRROLES
RECOVERY
REPAIR
RIBOSOMES
RODENTS
SULFONIC ACID ESTERS
TOXIC MATERIALS
TOXINS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology