Activation of protein kinase C by the lipid moieties of lipopolysaccharide
Protein kinase C (PKC) was partially purified from the RAW264.7 macrophage-like cell and characterized by its activation by phosphatidylserine (PS) in the presence of calcium and its insensitivity to cyclic nucleotides or calmodulin. This PKC can also be activated by the acidic lipid moieties of lipopolysaccharide (LPS). The LPS lipids activate PKC in the absence of PS and, like PS, synergize with diacylglycerol (DAG). Intact RAW264.7 cells were prelabelled with /sup 32/Pi and treated with the well characterized PKC ligands, phorbol myristate acetate (PMA) or DAG. The phosphoproteins thereby induced were separated in 2-D gels and visualized by autoradiography. These phosphoproteins were used as standards to identify the PKC-associated phosphoproteins induced in these cells using other stimulators. The authors demonstrate that the LPS lipids as well as LPS itself induce the formation of phosphoproteins common to those induced by PMA or DAG. PMA, DAG, the LPS lipids, and LPS itself activate the RAW264.7 cell and stimulate the release of prostaglandin D/sub 2/ at the same concentration that stimulate new protein phosphorylation. These results suggest that the activation of PKC is an early event in the activation of the RAW264.7 macrophage by LPS.
- Research Organization:
- Merck Sharp and Dohme Research Labs., Rahway, NJ
- OSTI ID:
- 5447431
- Report Number(s):
- CONF-8604222-; TRN: 86-028567
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
PHORBOL ESTERS
BIOLOGICAL EFFECTS
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
PURIFICATION
PROTEINS
PHOSPHORYLATION
AUTORADIOGRAPHY
CALCIUM
GLYCEROL
LIGANDS
LIPIDS
LIPOPOLYSACCHARIDES
MACROPHAGES
PHOSPHORUS 32
SERINE
ALCOHOLS
ALKALINE EARTH METALS
AMINO ACIDS
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARBOXYLIC ACIDS
CARCINOGENS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELEMENTS
ENZYMES
ESTERS
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTOPES
LIGHT NUCLEI
METALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHAGOCYTES
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYSACCHARIDES
RADIOISOTOPES
SACCHARIDES
SOMATIC CELLS
TRANSFERASES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
550201 - Biochemistry- Tracer Techniques