Four phosphoproteins with common amino termini are encoded by human cytomegalovirus AD169
In this report, the authors identify the proteins encoded by the 2.2-kilobase class of early transcripts arising from a region of the strain AD169 human cytomegalovirus genome (map units 0.682 to 0.713) which contains cell-related sequences. These transcripts, encoded by adjacent EcoRI fragments R and d, have a complex spliced structure with 5' and 3' coterminal ends. Antiserum directed against a synthetic 11-amino-acid peptide corresponding to the predicted amino terminus of the proteins was generated and found to immunoprecipitate four-infected-cell proteins of 84, 50, 43, and 34 kilodaltons. These proteins were phosphorylated and were associated predominantly with the nuclei of infected cells. The 43-kilodalton protein was the most abundant of the four proteins, and its level of expression remained relatively constant throughout the infection. Expression of the other proteins increased as the infection progressed. Pulse-chase analysis failed to show a precursor-product relationship between any of the proteins. A comparison of the (/sup 35/S)methionine-labeled tryptic peptide maps of the four proteins from infected cells and an in vitro-generated polypeptide derived from the putative first exon showed that all four infected-cell proteins were of viral origin and contained a common amino-terminal region.
- Research Organization:
- Univ. of California, San Diego, La Jolla
- OSTI ID:
- 5446277
- Journal Information:
- J. Virol.; (United States), Vol. 62:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PROTEINS
PHOSPHORYLATION
VIRUSES
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
AMINO ACID SEQUENCE
FIBROBLASTS
IMMUNE SERUMS
MAN
RNA
SULFUR 35
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
ISOTOPES
LIGHT NUCLEI
MAMMALS
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PRIMATES
RADIOISOTOPES
SOMATIC CELLS
SULFUR ISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques