Increase in the activities of glycolytic enzymes in rat lungs produced by nitrogen dioxide
Male Jcl: Wistar rats were exposed to 2, 4, and 10 ppm NO/sub 2/ for 14, 10, and 7 d, respectively, to examine the effect of NO/sub 2/ on the lung glycolytic pathway, a major energy-generating system in the lung. A highly significant increase in the activities of hexokinase, phosphofructokinase, 3-phosphoglycerate kinase, pyruvate kinase (PK), and lactate dehydrogenase was observed after 5 d exposure to 10 ppm NO/sub 2/, and a significantly higher value was maintained until d 7. Similarly, the activities of all enzymes examined increased significantly by exposure to 4 ppm NO/sub 2/, reaching the maximum between 4 and 7 d of exposure, and then approached to near the control levels. The most remarkable increase was found in the PK activity, which reached 1.82- and 1.53-fold that of the control at d 5 (10 ppm) and d 7 (4 ppm) of exposure, respectively. Upon exposure to 2 ppm NO/sub 2/, the PK activity of exposed animals was also increased to 1.23-fold that of the control at d 7, and higher activity was maintained until d 14. The glucose-6-phosphate dehydrogenase activity of exposed animals increased significantly at d 3, 4, and 14 of exposures to 10, 4, and 2 ppm NO/sub 2/, respectively, and a significantly higher value was maintained in the following period of exposure. These results show that short-term exposure of rats to 2-10 ppm NO/sub 2/ induces the pulmonary systems concerning glycolysis and NADPH-generation. The generation of energy and NADPH in the lung may be enhanced by NO/sub 2/ inhalation.
- Research Organization:
- National Institute for Environmental Studies, Yatabe, Japan
- OSTI ID:
- 5443225
- Journal Information:
- J. Toxicol. Environ. Health; (United States), Vol. 15:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
NADP
ENZYME ACTIVITY
NITROGEN DIOXIDE
TOXICITY
OXIDOREDUCTASES
PHOSPHOTRANSFERASES
BIOLOGICAL PATHWAYS
EXPERIMENTAL DATA
GLYCOLYSIS
INHALATION
LUNGS
METABOLISM
RATS
ANIMALS
BODY
CHALCOGENIDES
CHEMICAL REACTIONS
COENZYMES
DATA
DECOMPOSITION
ENZYMES
INFORMATION
INTAKE
MAMMALS
NITROGEN COMPOUNDS
NITROGEN OXIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
RESPIRATORY SYSTEM
RODENTS
TRANSFERASES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)