Complete physical map of the human immunoglobulin heavy chain constant region gene complex

Journal Article · Sat Jul 01 00:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.14.5567· OSTI ID:5442732

Hofker, M H; Walter, M A; Cox, D W ^[1]

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1. The Hospital For Sick Children, Toronto, Ontario (Canada)

The authors have found by pulsed-field gel electrophoresis that the human immunoglobulin heavy chain constant region gene complex maps entirely to a 350-kilobase (kb) Mlu I fragment. The enzyme Eag I was used with pulsed-field gel electrophoresis alone and in double digests with Spe I to map the region. C{sub {gamma}}3 maps 60 kb to the 3{prime} side of C{sub {delta}}; C{gamma}2 maps 80 kb to the 3{prime} side of C{sub {alpha}}1. C{sub {psi}{gamma}} maps 35 kb to the 3{prime} side of C{sub {alpha}}1 and is in the same transcriptional orientation as the other genes. Although in the cloned DNA many CpG-containing restriction sites were identified, most of these were methylated in peripheral blood leukocytes. The sites that were not methylated were predominantly found in three clusters, or Hpa I tiny fragment islands. A region showing strong linkage disequilibrium between all C{sub {gamma}} genes spans at least 160 kb. The 70-kb C{sub {mu}}-C{sub {gamma}}3 region, however, shows no linkage disequilibrium, possibly indicating a recombination hot spot. The immunoglobulin heavy chain constant region has been almost entirely cloned and mapped, and thus most rearrangements occurring in this region should be detectable.

• OSTI ID: 5442732
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:14; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA
AUTORADIOGRAPHY
IMMUNOGLOBULINS
GENETIC MAPPING
DNA-CLONING
ELECTROPHORESIS
PHOSPHORUS 32
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CLONING
DAYS LIVING RADIOISOTOPES
DNA HYBRIDIZATION
GLOBULINS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAPPING
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
550401* - Genetics- Tracer Techniques