Interactions between ADH and prostaglandins in isolated erythrocyte-perfused rat kidney

Journal Article · Sun Feb 01 00:00:00 EST 1987 · Am. J. Physiol.; (United States)

OSTI ID:5442246

Lieberthal, W; Vasilevsky, M L; Valeri, C R; Levinsky, N G

Interactions between antidiuretic hormone (ADH) and renal prostaglandins in the regulation of sodium reabsorption and urinary concentrating ability were studied in isolated erythrocyte-perfused rat kidneys (IEPK). In this model, hemodynamic characteristics are comparable to those found in vivo, and tubular morphology is preserved throughout the period of perfusion. (Deamino)-D-arginine vasopressin (dDAVP) markedly reduced fractional sodium excretion (FE/sub Na/) in the IEPK. After indomethacin, FE/sub Na/ fell still further. In the absence of dDAVP indomethacin had no effect on sodium excretion. dDAVP increased urine osmolality in the IEPK. When prostaglandin synthesis was blocked with indomethacin, urinary osmolality increased further. In isolated kidneys perfused without erythrocytes (IPK), dDAVP decreased FE/sub Na/ from 14.5 +/- 1.8% to 9.6 +/- 1.2%. dDAVP increased urine osmolality only modestly in the IPK and indomethacin did not increase concentrating ability further. Thus the IEPK (unlike the IPK) can excrete markedly hypertonic urine in response to ADH. ADH also enhances tubular reabsorption of sodium in the IEPK. Prostaglandins inhibit both these actions of ADH but do not directly affect sodium excretion in the absence of the hormone. Prostaglandius were measured by radioimmunoassay.

• Research Organization: Boston Univ. Medical Center, MA
• OSTI ID: 5442246
• Journal Information: Am. J. Physiol.; (United States), Vol. 252:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
KIDNEYS
PHYSIOLOGY
PEPTIDE HORMONES
BIOCHEMISTRY
PROSTAGLANDINS
RADIOIMMUNOASSAY
ERYTHROCYTES
EXCRETION
PERFUSED TISSUES
RATS
TRITIUM COMPOUNDS
URINE
VASOPRESSIN
VOLUME
ANIMAL TISSUES
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHEMISTRY
CLEARANCE
HORMONES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
ORGANS
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOLOGY
RODENTS
TISSUES
TRACER TECHNIQUES
VERTEBRATES
WASTES
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