Increased (/sup 125/I)trypsin-binding in serum from cystic fibrosis patients

Journal Article · Fri Jan 01 00:00:00 EST 1982 · J. Pediatr. Gastroenterol. Nutr.; (United States)

DOI:https://doi.org/10.1097/00005176-198201030-00011· OSTI ID:5441879

Cox, K L; Frates, Jr, R C; Sheikholislam, B M; Iwahashi-Hosoda, C K

The capacities of normal and cystic fibrosis (CF) sera to bind to exogenous human (/sup 125/I)trypsin were compared. Sera from eight older CF patients bound significantly more exogenous human (/sup 125/I)trypsin than did sera from eight normal subjects (p less than 0.001). Disregarding the increased trypsin-binding (TB) of CF sera, serum immunoreactive trypsinogen (SIRT) levels were not detectable in these eight older CF patients. However, when SIRT levels were corrected for TB, four CF patients had normal SIRT concentrations and four had low but detectable SIRT levels. As compared to five normal newborns' sera, serum from a newborn with CF had normal TB and the SIRT levels were very high. In conclusion, increased TB in CF serum lowers results of SIRT assays. Therefore, unless SIRT levels are corrected for TB, results obtained from currently available SIRT kits may be invalid.

• Research Organization: Department of Pediatrics, University of California Davis Medical Center, Sacramento
• OSTI ID: 5441879
• Journal Information: J. Pediatr. Gastroenterol. Nutr.; (United States), Vol. 1:3
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
TRYPSIN
RADIOIMMUNOASSAY
BLOOD CHEMISTRY
BLOOD SERUM
IODINE 125
LABELLED COMPOUNDS
METABOLIC DISEASES
PATIENTS
TRACER TECHNIQUES
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
PEPTIDE HYDROLASES
RADIOASSAY
RADIOISOTOPES
SERINE PROTEINASES
550601* - Medicine- Unsealed Radionuclides in Diagnostics