Lysis of cells infected with typhus group rickettsiae by a human cytotoxic T cell clone

Journal Article · Tue Dec 15 00:00:00 EST 1987 · J. Immunol.; (United States)

OSTI ID:5441828

Carl, M; Robbins, F; Hartzman, R J; Dasch, G A

Cytolytic human T cells clones generated in response to the intracellular bacterium Rickettsia typhi were characterized. Growing clones were tested for their ability to proliferate specifically in response to antigens derived from typhus group rickettsiae or to lyse targets infected with R. typhi or Rickettsia prowazekii, as measured by /sup 51/Cr-release from target cells. Two clones were able to lyse targets infected with typhus group rickettsiae. One of these clones was more fully characterized because of its rapid growth characteristics. This cytolytic clone was capable of lysing an autologous infected target as well as a target matched for class I and II histocompatibility leukocyte antigens (HLA). It was not capable, however, of lysing either a target mismatched for both class I and II HLA or a target partially matched for class I HLA. In addition, the clone exhibited specificity in that it was able to lyse an autologous target infected with typhus group rickettsiae, but did not lyse an autologous target infected with an antigenically distinct rickettsial species, Rickettsia tsutsugamushi. These results demonstrate, for the first time, that cells infected with intracellular bacteria can be lysed by human cytotoxic T lymphocytes.

• Research Organization: Naval Medical Research Institute, Bethesda, MD
• OSTI ID: 5441828
• Journal Information: J. Immunol.; (United States), Vol. 139:12
• Country of Publication: United States
• Language: English

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SECRETION
CLONE CELLS
IMMUNOLOGY
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LABELLED COMPOUNDS
MAMMALS
MATERIALS
MICROORGANISMS
NUCLEI
NUCLEOSIDES
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ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
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PRIMATES
PYRIMIDINES
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RIBOSIDES
RICKETTSIAL DISEASES
SOMATIC CELLS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
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