Glutathione and GSH-dependent enzymes in bronchoalveolar lavage fluid cells in response to ozone

Journal Article · Sat Feb 01 00:00:00 EST 1992 · Experimental and Molecular Pathology; (United States)

DOI:https://doi.org/10.1016/0014-4800(92)90021-3· OSTI ID:5440947

Boehme, D S; Hotchkiss, J A; Henderson, R F ^[1]

---

1. Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute, Albuquerque, NM (United States)

The purpose of this study was to determine if in vivo ozone exposure results in elevations in the levels of glutathione and glutathione-dependent enzymes in cells derived from bronchoalveolar lavage fluid (BALF). Our hypothesis was that, as part of a defense mechanism against oxygen toxicity, such cells would have increased levels of glutathione (GSH) in response to an oxidant stress. Female F344/N rats were exposed to 0.8 ppm ozone, 6 hr/day, for 1, 3, or 7 days, after which cells were collected by lung lavage. The GSH and GSH-peroxidase activity per milligram of protein in the cellular fraction, both necessary for reducing cellular peroxides, were elevated after 3 days of ozone exposure. After 7 days of exposure, cellular GSH had returned to control values, but the activity of glutathione reductase, the enzyme that reduces oxidized glutathione to GSH, was increased. Extracellular GSH concentration and glutathione reductase activity in BALF were also increased after 7 days of exposure. The total glutathione equivalents (GSH and GSSG, both cellular and extracellular) in BALF increased throughout the 7-day exposure, with GSH increasing first in the cells, and then in the extracellular fluid. This study demonstrated that the glutathione anti-oxidant system of BALF cells is stimulated by exposure to ozone. This response may serve to protect cells from the toxic effects of oxidant stress.

• OSTI ID: 5440947
• Journal Information: Experimental and Molecular Pathology; (United States), Vol. 56:1; ISSN 0014-4800
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
GLUTATHIONE
BIOSYNTHESIS
LUNGS
SENSITIVITY
OZONE
TOXICITY
ANIMAL CELLS
ENZYME ACTIVITY
INHALATION
LAVAGE
LIQUID COLUMN CHROMATOGRAPHY
OXIDOREDUCTASES
RATS
ANIMALS
BODY
CHROMATOGRAPHY
DRUGS
ENZYMES
INTAKE
MAMMALS
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RESPIRATORY SYSTEM
RODENTS
SEPARATION PROCESSES
SYNTHESIS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology