Ozone-initiated changes in erythrocyte membrane and loss of deformability

Journal Article · Mon Feb 01 00:00:00 EST 1988 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/S0013-9351(88)80013-6· OSTI ID:5440263

Morgan, D L; Furlow, T L; Menzel, D B

In vivo O/sub 3/ exposure followed by in vitro incubation was observed to cause inhibition of mouse RBC deformability. The requirement for in vitro incubation to allow expression of these effects and the potential role for oxidizable membrane components were investigated. Membrane sulfhydryls (SHs) and membrane ATPase are both susceptible to oxidation by O/sub 3/ and are essential for maintaining RBC membrane deformability. RBC SH levels and ATPase activity were unchanged immediately after exposure of mice to filtered air (controls) or 1 ppm O/sub 3/. After a subsequent 6-hr in vitro incubation, RBCs from control mice exhibited significant increases in membrane SH and ATPase activity, while SH levels and ATPase activity in RBCs from O/sub 3/-exposed mice remained unchanged. Although the stimulus for increasing membrane SH and ATPase activity is unclear, these changes appear to be essential to maintaining RBC deformability in vitro and are inhibited by in vivo O/sub 3/ exposure.

• Research Organization: Duke Univ. Medical Center, Durham, NC (USA)
• OSTI ID: 5440263
• Journal Information: Environ. Res.; (United States), Vol. 45:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ATP-ASE
ENZYME ACTIVITY
ERYTHROCYTES
MORPHOLOGICAL CHANGES
OZONE
BIOLOGICAL EFFECTS
CELL MEMBRANES
HEMOLYSIS
MICE
VITAMIN E
ACID ANHYDRASES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
ENZYMES
HYDROLASES
LYSIS
MAMMALS
MATERIALS
MEMBRANES
PATHOLOGICAL CHANGES
PHOSPHOHYDROLASES
RODENTS
VERTEBRATES
VITAMINS
560300* - Chemicals Metabolism & Toxicology