Affinity crosslinking of /sup 125/I-human beta-endorphin to cell lines possessing either mu or delta type opioid binding sites

Journal Article · Wed Jan 01 00:00:00 EST 1986 · NIDA Res. Monogr.; (United States)

OSTI ID:5437039

Keren, O; Gioannini, T L; Hiller, J M; Simon, E J

Affinity crosslinking of human /sup 125/I-beta-Endorphin to cell lines possessing either mu or delta binding sites was carried out. Autoradiography of SDS-PAGE gels from these crosslinked cell lines revealed that these two sites contain major peptide subunits that differ in molecular size. This confirms our earlier finding in mammalian brain which demonstrated separate and distinct subunits for mu and delta opioid receptors.

• Research Organization: New York Univ. Medical Center, NY (USA)
• OSTI ID: 5437039
• Journal Information: NIDA Res. Monogr.; (United States), Vol. 75
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ENDORPHINS
CROSS-LINKING
RECEPTORS
AFFINITY
AUTORADIOGRAPHY
CELL MEMBRANES
ELECTROPHORESIS
IODINE 125
MOLECULAR WEIGHT
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
CELL CONSTITUENTS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MEMBRANE PROTEINS
MEMBRANES
NEUROREGULATORS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
POLYMERIZATION
PROTEINS
RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques