Relative rates of albumin equilibration in the skin interstitium and lymph during vasodilation
The initial equilibration of /sup 125/I-labeled albumin between the vascular and extravascular compartments was studied in hindpaw skin of 6 anesthetized rabbits. Papavarine (200 ug/min) was infused into a small branch of the femoral artery of one limb with the contralateral limb as a control. There was a 1.2-fold increase in lymph flow (p < 0.01) with no significant change in the lymph-to-plasma total protein concentration ratio from prepopliteal lymphatics following papavarine. After reaching a constant, elevated lymph flow, tracer labeled albumin was infused to maintain the plasma activity constant for 3 hrs. The plasma volume in tissue samples was measured using /sup 131/I-labeled albumin injected 10 min before ending the experiment. Endogenous albumin was measured in plasma, lymph, and tissue samples using rocket electroimmunoassay. After 3 hrs of tracer infusion, lymph specific activity relative to plasma was significantly greater in the vasodilated hindlimb (0.30 +/- 0.07 vs 0.13 +/- 0.05; mean +/- SE; p < 0.01). Extravascular specific activity relative to plasma was greater in the vasodilated limb (0.13 +/- 0.02 vs 0.09 +/- 0.02; p < 0.05). Thus, vasodilation increased the rates at which lymph and tissue equilibrate with plasma. Also, the difference between lymph and tissue equilibration was greater in the vasodilated hindlimb.
- Research Organization:
- Albany Medical College, NY
- OSTI ID:
- 5436179
- Report Number(s):
- CONF-8604222-; TRN: 86-026616
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
Pulmonary transvascular flux of transferrin
Albumin and IgG in skin and skeletal muscle after plasmapheresis with saline loading
Related Subjects
ALBUMINS
EQUILIBRIUM
LABELLING
ARTERIES
VASODILATION
LYMPH
FLOW RATE
IODINE 125
IODINE 131
PROTEINS
RABBITS
SKIN
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD VESSELS
BODY
BODY FLUIDS
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
RADIOISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques