Comparison of histamine and hyperosmotic arabinose infusion on brain capillary permeability to hydrophilic solutes

Conference · Sat Mar 01 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5436167

Lucchesi, K J

The effect of bilateral intracarotid infusion of histamine (HA) on capillary permeability-surface area products (PS) of two metabolically inert tracers was determined and compared to that of L(+)arabinose (ARAB) in rat brain. Ringer's solution alone, or with 1 mg/kg HA diphosphate or 1.6M ARAB added, was infused (0.9 ml over 0.5 min) into each external carotid artery (CA). Five minutes later, a bolus of /sup 14/C-sucrose and /sup 3/H-L-glucose was injected i.v. Estimates of PS for both tracers were computed by the method of Ohno et al after brain concentration was corrected for tracer within cerebral blood vessels. Brain blood volume, based on the /sup 14/C-dextran space, was the same (.016 ml/g) in discrete cortical and midbrain regions of all rats except those treated with ARAB. The latter yielded .033 ml/g, presumably due to dextran extravasation. Infusion of ARAB, HA and Ringer's increased the PS's of sucrose and L-glucose by 10x, 8x, and 3x in brain regions perfused by the internal CA's. The ratio, PS-sucrose/PS-L-glucose was unchanged by any treatment. Both ARAB and HA caused transient falls in arterial pressure, but only ARAB caused deaths (3 of 9 rats). While as effective as ARAB in opening the blood-brain barrier, HA may be safer than hyperosmotic shock to enhance delivery of chemotherapeutic agents to brain tumors.

• Research Organization: Dartmouth Medical School, Hanover, NH
• OSTI ID: 5436167
• Report Number(s): CONF-8604222-; TRN: 86-026617
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ARABINOSE
BIOLOGICAL EFFECTS
CAPILLARIES
PERMEABILITY
HISTAMINE
BLOOD-BRAIN BARRIER
BRAIN
CARBON 14 COMPOUNDS
COMPARATIVE EVALUATIONS
GLUCOSE
INFUSION
PERFUSED ORGANS
RATS
SACCHAROSE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALDEHYDES
AMINES
ANIMALS
AZOLES
BLOOD VESSELS
BODY
CARBOHYDRATES
CARDIOVASCULAR SYSTEM
CENTRAL NERVOUS SYSTEM
DISACCHARIDES
HETEROCYCLIC COMPOUNDS
HEXOSES
IMIDAZOLES
INTAKE
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MONOSACCHARIDES
NERVOUS SYSTEM
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PENTOSES
RODENTS
SACCHARIDES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques