Destabilization of simple repetitive DNA sequences by transcription in yeast

Journal Article · Sat Jun 01 00:00:00 EDT 1996 · Genetics

OSTI ID:543011

Wierdl, M; Petes, T D ^[1]; Jinks-Robertson, S ^[2]

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1. Univ. of North Carolina, Chapel Hill, NC (United States)
2. Emory Univ., Atlanta, GA (United States); and others

Simple repetitive DNA sequences in the eukaryotic genome frequently alter in length. In wild-type strains, we find that transcription through a repetitive poly GT tract destabilizes the tract four- to ninefold. In mismatch repair-deficient yeast strains, simple repeats are very unstable. High levels of transcription in such strains destabilize repetitive tracts an additional two- to threefold. 44 refs., 2 figs., 4 tabs.

• OSTI ID: 543011
• Journal Information: Genetics, Vol. 143, Issue 2; Other Information: PBD: Jun 1996
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
DNA
DNA REPAIR
DNA SEQUENCING
LENGTH
STRUCTURE-ACTIVITY RELATIONSHIPS
DNA REPLICATION
MUTATIONS
YEASTS
STRAINS
DNA POLYMERASES
CHROMOSOMES
CROSSING-OVER
SISTER CHROMATID EXCHANGES
GENETIC MAPPING
HUMAN POPULATIONS
HEREDITARY DISEASES
GENES
TRANSCRIPTION
GENE RECOMBINATION
PLASMIDS
NUCLEOTIDES