Hepatocyte handling of immunoglobulin A in the rat: the role of microtubules

Journal Article · Fri Jul 01 00:00:00 EDT 1983 · Gastroenterology; (United States)

OSTI ID:5429246

Goldman, I S; Jones, A L; Hradek, G T; Huling, S

Plasma-derived dimeric immunoglobulin A is transported through liver parenchymal cells into bile, in association with its glycoprotein receptor secretory component, by a vesicular transport system. This study was designed to determine the effects of colchicine, a microtubule-disrupting agent, and thus the role of microtubules on the uptake, intracellular transport, and subsequent biliary secretion of dimeric immunoglobulin A. In vivo studies in rats showed that colchicine treatment reduced the amount of intraportally injected /sup 125/I-dimeric immunoglobulin A that appeared in the bile. It was also found that although the livers in colchicine-treated animals could sequester and internalize immunoglobulin A, it was not readily secreted into bile. In vitro studies using peroxidase-labeled antisecretory component and /sup 125/I-dimeric immunoglobulin A autoradiography were both used to determine the site of this block in immunoglobulin A secretion. These studies demonstrate that colchicine disruption of microtubules (a) has little initial effect on the binding and internalization of dimeric immunoglobulin A; (b) has a major effect on the translocation of immunoglobulin A-containing vesicles within the hepatocyte, and (c) most likely prevents the translocation of newly synthesized secretory component to the plasma membrane.

• Research Organization: Cell Biology and Aging Section, Veterans Administration Medical Center, San Francisco, California
• OSTI ID: 5429246
• Journal Information: Gastroenterology; (United States), Vol. 85:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
IMMUNOGLOBULINS
TRANSLOCATION
LIVER
PHYSIOLOGY
ANIMAL CELLS
BILE
BLOOD PLASMA
COLCHICINE
IODINE 125
LABELLED COMPOUNDS
RATS
TRACER TECHNIQUES
ALKALOIDS
ANIMALS
ANTIMITOTIC DRUGS
ANTIPYRETICS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
GLOBULINS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RODENTS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques