Renal catabolism of /sup 125/I-glicentin

Journal Article · Thu May 01 00:00:00 EDT 1986 · Am. J. Physiol.; (United States)

OSTI ID:5429162

Lopez-Novoa, J M; Santos, J C; Villamediana, L M; Garrote, F J; Thim, L; Moody, A J; Valverde, I

The renal catabolism of /sup 125/I-glicentin has been studied in vivo by the disappearance of this peptide from the plasma of bilaterally nephrectomized, ureteral-ligated, or normal rats and by using tubular microinfusion techniques. In addition the catabolism of glicentin by the isolated, perfused kidney has been studied. Results from in vivo studies demonstrated that half-disappearance time was lower in control (59.5 +/- 1.8 min) than in bilaterally nephrectomized rats (97.2 +/- 2.6 min), and this value was significantly higher than that of ureteral-ligated animals (83.2 +/- 1.1 min, P less than 0.005). Microinfusion experiments revealed that when /sup 125/I-glicentin was injected into the proximal tubule, no trichloroacetic-precipitable radioactivity was recovered in the urine, whereas most of inulin injected was recovered. By contrast most of the /sup 125/I-glicentin injected into the distal tubule was recovered in the urine. In isolated kidney experiments, organ clearance rate of /sup 125/I-glicentin averaged 0.88 +/- 0.10 ml/min, a value significantly higher than that of glomerular filtration rate (0.72 +/- 0.06 ml/min, P less than 0.005, paired data), and both parameters showed a close linear relationship (r = 0.90). Urinary clearance of glicentin was negligible. These results demonstrate that the kidney plays a major role in the catabolism of glicentin, mainly by glomerular filtration and tubular catabolism. The site of tubular catabolism appears to be the proximal tubule. Peritubular uptake was minimal.

• Research Organization: Fundacion Jimenez Diaz, Madrid, Spain
• OSTI ID: 5429162
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PEPTIDES
CATABOLISM
RENAL CLEARANCE
BIOCHEMICAL REACTION KINETICS
GLUCAGON
IN VITRO
IODINE 125
KIDNEYS
NEPHRECTOMY
PERFUSED ORGANS
PRECIPITATION
RATS
TRACER TECHNIQUES
URINE
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
BODY FLUIDS
CLEARANCE
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EXCRETION
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MATERIALS
MEDICINE
METABOLISM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SEPARATION PROCESSES
SURGERY
VERTEBRATES
WASTES
550501* - Metabolism- Tracer Techniques