Use of a solid-state multihead gamma counter in a second-generation system for solid-phase immunoassay
Simultaneous advances in detector technology and solid-phase separation systems, as well as the availability of powerful desktop computers, have made possible the development of ''second-generation'' solid-phase immunoassays. These retain the advantages of classical solid phase while significantly accelerating reaction kinetics. Hapten assays--such as for digoxin, thyroxin, and triiodothyronine uptake--in batches of 48 are processed in about 20 min from reagent introduction until hard-copy printout, with minimal operator involvement. The system also functions as a 48-detector gamma counter, capable of counting and reducing data for any /sup 125/I-based RIA that can be run in a 12 X 75 mm test tube. System control, data management, and computer screen displays of kinetic data are provided by an unmodified Hewlett Packard HP-87XM computer. User-friendly disc-based software facilitates the creation and storage of counting and data reduction protocols for as many as 30 RIAs from various manufacturers as well as up to 30 of our own assays.
- Research Organization:
- Medical and Scientific Designs, Inc., Rockland, MA
- OSTI ID:
- 5428814
- Journal Information:
- Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 29:9
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
DIGOXIN
RADIOIMMUNOASSAY
THYROXINE
TRIIODOTHYRONINE
AUTOMATION
COMPUTER CALCULATIONS
COMPUTERS
GAMMA DETECTION
IODINE 125
REACTION KINETICS
AMINO ACIDS
BETA DECAY RADIOISOTOPES
CARBOHYDRATES
CARBOXYLIC ACIDS
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
DAYS LIVING RADIOISOTOPES
DETECTION
DIGITALIS GLYCOSIDES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GLYCOSIDES
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
PEPTIDE HORMONES
RADIATION DETECTION
RADIOASSAY
RADIOISOTOPES
THYROID HORMONES
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550201 - Biochemistry- Tracer Techniques