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Title: Evidence for human meiotic recombination interference obtained through construction of a short tandem repeat-polymorphism linkage map of chromosome 19

Abstract

An improved linkage map for human chromosome 19 containing 35 short tandem repeat polymorphisms (STRPs) and one VNTR (D19S20) was constructed. The map included 12 new (GATA)[sub n] tetranucleotide STRPs. Although total lengths of the male (114 cM) and female (128 cM) maps were similar, at both ends of the chromosome male recombination exceeded female recombination, while in the interior portion of the map female recombination was in excess. Cosmid clones containing the STRP sequences were identified and were positioned along the chromosome by fluorescent in situ hybridization. Four rounds of careful checking and removal of genotyping errors allowed biologically relevant conclusions to be made concerning the numbers and distributions of recombination events on chromosome 19. The average numbers of recombinations per chromosome matched closely the lengths of the genetic maps computed by using the program CRIMAP. Significant numbers of chromosomes with zero, one, two, or three recombinations were detected as products of both female and male meioses. On the basis of the total number of observed pairs of recombination events in which only a single informative marker was situated between the two recombinations, a maximal estimate for the rate of meiotic STRP [open quotes]gene[close quotes] conversion without recombination wasmore » calculated as 3 [times] 10[sup [minus]4]/meiosis. For distances up to 30 cM between recombinations, many fewer chromosomes which had undergone exactly two recombinations were observed than were expected on the basis of the assumption of independent recombination locations. This strong new evidence for human meiotic interference will help to improve the accuracy of interpretation of clinical DNA test results involving polymorphisms flanking a genetic abnormality. 61 refs., 2 figs., 5 tabs.« less

Authors:
; ; ; ; ; ;  [1];  [2];  [3]; ;  [4]
  1. Marshfield Medical Research Foundation, Marshfield, WI (United States)
  2. Louisiana State Univ. Medical Center, New Orleans, LA (United States)
  3. Massachusetts Institute of Technology, Cambridge, MA (United States)
  4. Lawrence Livermore National Lab., CA (United States)
Publication Date:
OSTI Identifier:
5428676
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Journal Article
Journal Name:
American Journal of Human Genetics; (United States)
Additional Journal Information:
Journal Volume: 53:5; Journal ID: ISSN 0002-9297
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GENE RECOMBINATION; INTERFERENCE; HUMAN CHROMOSOME 19; GENETIC MAPPING; MEIOSIS; RFLPS; CELL DIVISION; CHROMOSOMES; HUMAN CHROMOSOMES; MAPPING; 550400* - Genetics

Citation Formats

Weber, J L, Wang, Z, Hansen, K, Stephenson, M, Kappel, C, Salzman, S, Wilkie, P J, Keats, B, Dracopoli, N C, Brandriff, B F, and Olsen, A S. Evidence for human meiotic recombination interference obtained through construction of a short tandem repeat-polymorphism linkage map of chromosome 19. United States: N. p., 1993. Web.
Weber, J L, Wang, Z, Hansen, K, Stephenson, M, Kappel, C, Salzman, S, Wilkie, P J, Keats, B, Dracopoli, N C, Brandriff, B F, & Olsen, A S. Evidence for human meiotic recombination interference obtained through construction of a short tandem repeat-polymorphism linkage map of chromosome 19. United States.
Weber, J L, Wang, Z, Hansen, K, Stephenson, M, Kappel, C, Salzman, S, Wilkie, P J, Keats, B, Dracopoli, N C, Brandriff, B F, and Olsen, A S. 1993. "Evidence for human meiotic recombination interference obtained through construction of a short tandem repeat-polymorphism linkage map of chromosome 19". United States.
@article{osti_5428676,
title = {Evidence for human meiotic recombination interference obtained through construction of a short tandem repeat-polymorphism linkage map of chromosome 19},
author = {Weber, J L and Wang, Z and Hansen, K and Stephenson, M and Kappel, C and Salzman, S and Wilkie, P J and Keats, B and Dracopoli, N C and Brandriff, B F and Olsen, A S},
abstractNote = {An improved linkage map for human chromosome 19 containing 35 short tandem repeat polymorphisms (STRPs) and one VNTR (D19S20) was constructed. The map included 12 new (GATA)[sub n] tetranucleotide STRPs. Although total lengths of the male (114 cM) and female (128 cM) maps were similar, at both ends of the chromosome male recombination exceeded female recombination, while in the interior portion of the map female recombination was in excess. Cosmid clones containing the STRP sequences were identified and were positioned along the chromosome by fluorescent in situ hybridization. Four rounds of careful checking and removal of genotyping errors allowed biologically relevant conclusions to be made concerning the numbers and distributions of recombination events on chromosome 19. The average numbers of recombinations per chromosome matched closely the lengths of the genetic maps computed by using the program CRIMAP. Significant numbers of chromosomes with zero, one, two, or three recombinations were detected as products of both female and male meioses. On the basis of the total number of observed pairs of recombination events in which only a single informative marker was situated between the two recombinations, a maximal estimate for the rate of meiotic STRP [open quotes]gene[close quotes] conversion without recombination was calculated as 3 [times] 10[sup [minus]4]/meiosis. For distances up to 30 cM between recombinations, many fewer chromosomes which had undergone exactly two recombinations were observed than were expected on the basis of the assumption of independent recombination locations. This strong new evidence for human meiotic interference will help to improve the accuracy of interpretation of clinical DNA test results involving polymorphisms flanking a genetic abnormality. 61 refs., 2 figs., 5 tabs.},
doi = {},
url = {https://www.osti.gov/biblio/5428676}, journal = {American Journal of Human Genetics; (United States)},
issn = {0002-9297},
number = ,
volume = 53:5,
place = {United States},
year = {Mon Nov 01 00:00:00 EST 1993},
month = {Mon Nov 01 00:00:00 EST 1993}
}